EXPERIMENTAL. 49 



Twenty grams of germ meal were extracted with 500 cc. of 3 per cent 

 sodium-chloride solution heated to 70 , whereby the leucosin was coagu- 

 lated and the globulin and proteose dissolved. Of the clear filtered extract 

 100 cc. yielded with tannin a precipitate containing 0.0166 gram nitrogen 

 per gram of meal extracted. 



Two portions of the meal, each of 1 gram, were exhausted with 3 per 

 cent sodium-chloride solution heated to yo and nitrogen determined in the 

 residues. The 0.0331 gram and 0.0309 gram of nitrogen found in the resi- 

 dues were from the leucosin and insoluble nitrogenous bodies, so that the 

 nitrogen belonging to the latter equaled 0.0171 gram and 0.0149 gram. 

 From the average of these figures we find the following amounts of the 

 different forms of nitrogen in 1 gram of the wheat-germ meal : 



N, grains. 



Insoluble in water and salt solution 0160 



Insoluble in water, but soluble in salt solution (globulin nitrogen) . . .0100 



Soluble in water and coagulable by heat (albumin nitrogen) 0160 



Soluble in water, uncoagulable by heat, precipitable by tannin (pro- 

 teose nitrogen) 0050 



Not precipitable by tannin (non-protein nitrogen) 0060 



Total 0530 



Found by direct nitrogen determination 0531 



It has been shown that the coagulated leucosin preparations contain about 

 10 per cent of nucleic acid, the globulin about 15 per cent, while those of 

 the proteose contain none. Deducting these quantities from the nitrogen 

 given above, it is found that 9.5 percent of the embryo is leucosin, 4.84 per 

 cent globulin, and 3.03 per cent proteose. 



The bodies which are represented by the insoluble nitrogen could not be 

 separated from the embryo. The residue, after extraction with hot-salt 

 solution, contained 0.0076 gram of phosphorus. In view of the large 

 proportion of nucleic acid found in the extracts of the embryo, it is not 

 improbable that this phosphorus mostly belongs to nucleic acid, and that the 

 insoluble nitrogen largely belongs to compounds of protein with relatively 

 much nucleic acid. 



DIGKSTION OF THE PHOSPHORUS-CONTAINING PROTEIN PREPARATIONS 

 WITH PEPSIN-HYDROCHEORIC ACID. 



IvEucosin Nucleate. 



Ten grams of the coagulated albumin, preparation S, were suspended in 

 400 cc. of water and dissolved by adding 100 cc. of decinormal potassium- 

 hydroxide solution. To the nearly clear solution which resulted an equal 

 volume of 0.4 per cent hydrochloric acid was added, together with some 

 pepsin, and the mixture digested at 37 . In a short time the solution 

 4 



