EXPERIMENTAL. 



57 



If the preceding analyses are brought together as in table 13, the effect 

 of the various fractional solutions and precipitations may be seen at a glance. 



It is evident that 58 and 59 contain less nitrogen than the great bulk of 

 the protein extracted. This is due to fat which they were found to contain 

 and which could not be wholly removed by extraction with ether, since in 

 drying these preparations were converted into the horny condition which 

 rendered penetration with ether impossible. 



Preparation 67 was evidently impure, as might be expected, since it con- 

 tained all of the insoluble particles of the entire extract which had escaped 

 nitration, and owing to its small amount these impurities produced a marked 

 effect on its percentage composition. The same is true of 68, but as the 

 quantity of this preparation is so much greater this contamination has pro- 

 duced much less effect on its composition. Preparation 65 was obtained by 

 evaporating the mother-liquor from 64 nearly to dryness and then extracting 

 with absolute alcohol and ether. It would hardly be expected that under 



