EXPERIMENTAL. 



8 9 



analyzed, preparation 102 was found to have the composition shown in the 

 table on page 88. 



The filtrate from this preparation, as well as all the others previously- 

 described, contained a small amount of protein matter. This was then 

 treated with a solution of copper sulphate and the small precipitate thereby- 

 produced filtered off, washed with water, alcohol, and ether (preparation 

 103), and found to contain the following amount of nitrogen : 



Preparation 103. 



In the same way as 102 a preparation of this protein was made from 

 flour from the entire kernel of winter wheat. The composition of this 

 preparation is shown by the figures in the following table for preparation 

 104. 



Preparations 104 and 105. 



A second portion of the same solution from which preparation 104 had 

 been precipitated was obtained 2 days later. This was then treated in 

 the same way and yielded preparation 105, which was found to have the 

 composition shown in the table above. 



If preparations 89 and 92 are omitted on account of being obtained from 

 unfiltered solutions, 94 as an altered and insoluble product, and 95 as having 

 been subjected to an exceedingly prolonged action of an alkaline solution, 

 then will be had, in table 18, the analyses which, in the opinion of the 

 writer, most nearly represent the true composition of this protein. 



