94 



THE PROTEINS OF THE WHEAT KERNI'J.. 



These esters were saponified by boiling with 6 parts of water for 5 hours, 

 when their solution reacted neutral to litmus. After evaporating to dryness 

 under reduced pressure, the dried residue was extracted with boiling absolute 

 alcohol. The part insoluble in alcohol, by a systematic fractional crystal- 

 lization, gave 32.42 grams of leucine, which decomposed at about 298 . 



Carbon and hydrogen: 0.4255 gram substance, dried at no , gave 0.8535 gram C0 2 



and 0.3926 gram H 2 0. 

 Nitrogen: 0.1488 gram substance gave NH,= 1.60 cc. HC1 (1 cc HCl = o.oi gram N). 

 Calculated for C G H 13 0,N, C 54.89, H 10.01, N 10.70 p. ct.; found, C 54.71, H 10.251 



N 10.77 P- ct - 



The filtrate from this leucine yielded 2 grams of substance, which had the 

 composition and properties of amino-valerianic acid. 



Carbon and hydrogen: 0.2216 gram substance gave 0.4144 gram C0 2 and 0.1967 gram 



H 2 0. 

 Calculated for C 5 H u 2 N, C 51.22, H 9.48 p. ct.; found, C 51.00, H 9.86 p. ct. 



Specific rotation. Dissolved in 20 per cent hydrochloric acid, 



0) -^ = + 25.63 



A similar preparation from gliadin gave +25.79 . E. Fischer & Dor- 

 pinghaus 1 found +25.9 for a preparation from horn, and E. Schulze & 

 Winterstein 2 found +28.2 and + 27.9 for a preparation from lupine 

 seedlings. 



The solution used for determining the specific rotation was freed from 

 hydrochloric acid with silver sulphate, and the amino-acids racemized by 

 heating with an excess of barium hydroxide in an autoclave at 175 for 19 

 hours. After removing the barium quantitatively with sulphuric acid, the 

 substance was coupled with a-naphthylisocyanate. The hydantoic acid 

 melted constantly, on repeated recrystallization from dilute alcohol, at 183 to 



'Fischer, E., & Dorpinghaus, Zeitschrift fur physiologische Chemie, 1902, xxxvi, 

 p. 462. 

 2 Schulze, E., & Winterstein, ibid., 1902, xxxv, p. 300. 



