EXPERIMENTAL. 101 



rides were removed, which required 5 days. The precipitated globulin was 

 then filtered from each, washed with distilled water, alcohol, absolute alcohol, 

 and ether, removed from the filter, and dried at no. From the spring- 

 wheat extract 3.8398 grams were obtained, equal to 0.624 per cent of the 

 flour, and from the winter wheat 3.9265 grams, equal to 0.625 per cent. 



The filtrates from the globulin were then heated to 65 in a water-bath, 

 and after being held at this temperature for some time the coagulum was 

 filtered off and washed with hot water, alcohol, and ether, removed from 

 the paper, and dried at no . From the spring wheat 1.97 14 grams were 

 obtained, being 0.315 per cent of the flour, and from the winter wheat 

 1.9614 grams, equal to 0.302 per cent. 



The solutions filtered from each of these coagula were next heated just 

 to boiling and the resulting coagulum filtered off, washed thoroughly, and 

 treated as the preceding preparations had been. The spring- wheat extract 

 thus yielded 0.4743 gram, equal to 0.076 percent ; the winter-wheat extract 

 0.3680 gram, equal to 0.057 P er cent. 



The two extracts were next concentrated by boiling down over a lamp. 

 They remained clear at first, but when somewhat concentrated the protein 

 began to separate as a skin on the surface of the solution. When reduced 

 to about one-fourth its original volume, the coagulum was filtered off, washed 

 with boiling water, alcohol, and ether, and dried at 1 io. The spring wheat 

 thus yielded 0.8737 g ram > equal to 0.139 per cent ; the winter wheat 0.8721 

 gram, equal to 0.134 per cent. The filtrates from these two preparations 

 were evaporated very nearly to dryness on water-baths. On cooling, much 

 substance separated, which, when treated with hot water, dissolved again. 

 The insoluble coagulum was filtered from each, washed, and dried in the 

 usual manner. The spring wheat gave 0.8149 gram of substance, being 

 0.130 per cent of the flour ; the winter wheat 0.5795 gram, being 0.089 P er 

 cent. The total amount of protein coagulating on concentration was there- 

 fore 0.269 P er ceQ t for the spring wheat and 0.223 per cent for the winter 

 wheat. 



The filtrates from these second coagula were then again evaporated to a 

 sirup, and as no more insoluble matter separated they were each precipitated 

 by pouring into strong alcohol. Large precipitates resulted in each case, 

 which, after settling and decantation of the alcohol, were dissolved again in 

 a little water and precipitated by pouring into strong alcohol. Much color- 

 ing matter and sugar was held in solution, as proved by evaporation of the 

 alcoholic mother-liquors. The precipitates were then thoroughly dehydrated 

 with absolute alcohol, washed with ether, and dried at no. The spring- 

 wheat extract thus yielded 6.9289 grams of substance, the winter wheat 

 8.7517 grams. As these preparations were unquestionably very impure 



