156 PROCEEDINGS OF THE ACADEMY OF [PaRT I 



made to occur at any of the phases of division, the second, third, 

 fourth, etc. 



The animalcules of the same generation are all alike, and of the 

 same size but after the third (or fourth?) division, they differ from 

 those which came from the first and second (perhaps also the third) 

 division in an important regard: they have, or they may have, a 

 flageJlum, 



In one of these small Flagellates, such as was born from the eighth 

 division (Plate VIII, fig. 61), the vermiform body is very clear and 

 still full of paramylum grains, but these are now very small; just 

 at the posterior extremity two or three larger globules are seen 

 (excretion granules), colorless and highly refractive. The hind- 

 part of the body is itself pointed, sometimes drawn out into a glutin- 

 ous thread-like tail. The nucleus is central, with the character- 

 istic granulations. The contractile vesicle and reservoir are quite 

 as we have already described, and from the bottom of the reservoir 

 are seen to arise two flagella; but while one of these remains in a 

 rudimentary state, just as it was in the big individuals, the other 

 projects through the buccal tube to the outside, rapidly beating, 

 and trailing the animalcule behind. This flagellum, however, is 

 very slender, and comes off on the least excuse; many of the speci- 

 mens do not have it, either having lost it already or never having 

 possessed it. 



According to Moroff (25), in his diagnosis of Euglena quartana, 

 a very near relative to Astasia, division takes place as follows: 

 "Division after loss of the flagella, either longitudinally into two 

 new individuals, or into four parts after acquiring the globular 

 condition." According to this observer, the second division^* is 

 at right angles to the first, and seems then to be transverse, the first 

 being longitudinal; but if we keep in mind that all the indications 

 of transverse division in Euglenae have been considered by Klebs 

 and others as very doubtful, we must hesitate in accepting Moroff's 

 statement. It is quite possible that even in the globular condition, 

 after a first division, the two segments insensibly move or displace, 

 and the next division, which is for the observer at right angles to 

 the first, i. e., transverse, is longitudinal in reality. In all circum- 

 stances where I could follow the process in its details, the division 

 proved to be longitudinal; and in some individuals, which though 

 already creeping about were yet in a state of division, one could 



1* Moroff never obtained more than two divisions, that is, four young indi- 

 viduals. 



