464 PROCEEDINGS OF THE ACADEMY OF [July, 



(2) A chromatin body. 



(3) A vacuole. 



(4) A chromatin body. 



These four elements are normal constituents of all of the spores, 

 although it is often difficult to see them all in any given spore. 



The chromatin bodies are probably nuclei, but this point will be 

 considered later. The vacuoles are not to be confounded with the 

 small vacuoles frequently present here, as in all other protozoa, but 

 are clearly morphological entities. 



For convenience, the vacuoles will be referred to as vacuole No. 1 

 and vacuole No. 2; the chromatin bodies as chromatin body No. 1 

 and chromatin body No. 2, the count being made from the broad to 

 the narrow end of the spore. 



Vacuole No. 1. — This, when distinct, appeared as a very narrow ring, 

 inclosing a clear space. In other cases it was not nearly so sharply 

 differentiated, appearing only as a vague, poorly defined region, while 

 very often it could not be seen at all. The space within the ring was 

 sometimes colorless, but more often faintlv stained, and at times took 



7 *7 7 



much the same stain as the surrounding cytoplasm. Occasionally, 

 it contained a faintly staining granule or irregularly shaped mass, 

 staining a trifle more deeply than the ground substance. In spores 

 still in situ within the compartments of the cyst the vacuole was 

 often very conspicuous, presenting the aspect of a hole in the cyto- 

 plasm. 



The vacuole was usually nearly or quite round. It was sometimes 

 at the extreme end of the spore ; in other cases some distance from the 

 end. It varied a good deal in size, at times being large enough to fill 

 the entire width of the cell, again quite small. The appearance is 

 shown in figs. 1-3, 10, 12. 



The different stains used made little or no difference in the appear- 

 ance of this element. 



It is possible that this is the so-called striated body said to be 

 present in sarcosporidian spores, and interpreted as the homologue 

 of the polar capsule of the spores of Myxosporidia. 



Chromatin Body No. 1. — The appearance of this element is shown 

 in figs. 1-3, 5, 6, 8 and 10-12. 



Viewed under a magnification of some 700-800, it appeared as a 

 solid, deeply stained, spherical, oval or roughly demi-lunar body, 

 frequently broken into two equal or unequal parts. (Plate NNNVI. 

 figs. 1-3, 5c, Sd, e, 10, 11). Under higher powers, however, and with 

 an intense light, it was frequently possible to make out that it was 



