1911.] NATURAL SCIENCES OF PHILADELPHIA. 465 



more or less lobulated, and apparently composed of several masses, 

 closely compacted together (fig. 5a, b, h). Much more rarely it 

 consisted of an aggregate of large granules rather than lobes (figs. 

 5d, f, 6b). 



Sometimes the appearances suggested division into two (figs. 13, 

 Sd, e). 



Generally speaking, this body took a dense chromatin stain, but 

 frequently it did not stain homogeneously, one part taking the chroma- 

 tin, the other the plasma stain. Thus, the case shown by fig. 13 

 shows two heavily stained demilunes, with between them some plasma- 

 like substance. Figs. 6c and 8a, b, c, show elements which are solid, 

 but which stain partly like chromatin and partly like plasma. It is 

 by no means unlikely that in cases where the element appears double 

 (fig. 8c/, e) the conditions really are as in fig. 86, the plasmic portion 

 having failed to stain. Further, in the case of such a body as is shown 

 in figs. 6c, 8a, b, c, an alteration in the chemical composition of the 

 central portion, or overstating, would give elements such as are shown 

 in figs. 1 and 2. 



The data just given suggest that this element is a nucleus of the 

 massive or compact type, such as the macronuclei of the ciliate Infu- 

 soria. It has ordinarily the aspect of a solid mass, but is composed 

 rather of several closely compacted masses. Under certain conditions 

 a larger or smaller portion of it changes in chemical composition and 

 stains like albumin. 



In smears fixed with Hermann's fluid this body frequently lay in a 

 vacuole, but it is believed that such an appearance is the result of a 

 too violent fixation. 



In smears stained with iron-hsematoxylin the color is sensibly 

 black. In thionin it is deep blue, while in Wright's stain it is a 

 nearly black garnet, precisely like the kinetonuclei of trypanosomes. 



Vacuole No. 2. — In general this is more distinct than vacuole No. 1 . 

 Usually, it showed as a distinct ring, which was frequently irregularly 

 thickened. At times, this vacuole has the appearance of a hole in 

 the cytoplasm, and this was always the case when the spores were 

 in situ; either in sections or in cyst compartments in the smears. 



But in many cases, it is inconspicuous (see figs. 1,3). Here it is 

 seen to be irregularly shaped and quite small. More probably, this 

 is an accident of fixation. This vacuole, in the spore, lies between 

 two chromatin bodies, and hence can easily be pressed upon during 

 fixation. In the spores in the cysts it is always very conspicuous, 

 and it was also quite evident in the fresh spores. 



