If)!) Beard, On the Occurrence of Dextro-rotatory Albumins in Organic Nature. 



experiments, using the same Fairchild preparations, and as I have 

 employed them, convince himself, that his contemplated contradiction 

 and refutation are futile. 



A condition of all the experiments is, that the micro-organisms 

 used should be in an active healthy state. It would, of course, be 

 feasible to carry out the experiments to a successful issue with 

 much smaller quantities of ferments, provided the experiments were 

 made at a higher temperature, for instance, at blood-heat or between 

 38 and 40 degrees Centigrade. It was, however, more convenient 

 to make the experiments at the ordinary temperature of a room, 

 and with such dilutions of the combined ferments, trypsin and 

 amylopsin (Fairchild), as would enable their deadly disintegrating 

 effects upon healthy living asexual micro-organisms to be observed, 

 as a rule, within the space of an hour or less. It is, be it remarked, 

 quite unnecessary for any critic to point out, that the finds recorded 

 here could have been obtained by the use of trypsin alone without 

 the aid of amylopsin. Of that fact I am aware. But the reason 

 why amylopsin was also invoked in the experiments was, because 

 I thought, and shall always think, that the use of trypsin alone in 

 the treatment of cancer, various tropical diseases, tuberculosis, etc. 

 is a very dangerous proceeding, even in many cases a deadly one. 

 I wished, therefore, to avoid doing anything to encourage a belief, 

 that in the practice of medicine trypsin without the aid of its com- 

 plement, amylopsin, could be regarded as a safe and efficacious 

 remedy. 



The reagents and the conditions under which they were used 

 were as follows. Since there are on the market preparations, even 

 injections for use in medicine, of pancreatic ferments, trypsin and 

 amylopsin, which are either excessively weak, or unreliable, or even 

 quite inert, it was necessary to take for the experiments such 

 injections of trypsin and amylopsin in combination as were strong, 

 of known strength, standardised, and of a stable character. As 

 W. Batzner has shown 6 ), the most powerful, reliable, and stable 

 pancreatic injections are the ones manufactured by Messrs Fair- 

 child Bros & Foster. To distinguish them from earlier and 

 weaker injections made by the same firm, they will be referred to 

 here as the Fairchild U 1912" injections of trypsin and amylopsin. 

 These injections are standardised in various ways, not in a single 

 one, and thus it is possible to refer to the trypsin-injection, for 

 example, as Batzner does, as possessing a potency in dilution of 

 1 : 4000, tested by the Jochmann method, or by the Roberts 



6) Batzner, W. Trypsiribehandlung der cbirurgischen Tuberkulose, in: Arch, 

 klin. Chirurgie, Vol.95, p. 5, 1911. 



idem. The Practitioner, Jan. 1913, p. 205. 



