258 STUDIES ON PATHOLOGIC OVA. 



give the entire wall of the cavity containing the ovum. The sections, showing 

 most of the structures, are at right angles to the ovary (directly through the letter 

 in the word ovary, figure 157). They were stained in a great variety of ways: 

 hematoxylin, acid fuchsin, iron hematoxylin, orange G, and a number of connective- 

 tissue stains. In general, they show that the ovary is active and not fibrous, 

 apparently normal, containing numerous blood-vessels and a ring of large Graa- 

 fian vesicles (figures 158 and 159), with an outside zone of small vesicles containing 

 small ova. To all appearances this is as it should be in a y-oung ovary. 



The wall of the cavity, containing the blood-clot and villi, is lined almost 

 throughout with a layer of lutein cells (figure 161). This layer is quite uniform, 

 ranging from 0.5 to 1 mm. in thickness. Between the lutein cells are numerous 

 strands of blood-vessels, but on their inner side is a layer of fibrin before the blood- 

 clot begins. On the outside the lutein cells form small islands of more compact 

 cells which stain more intensely in hematoxylin (figure 161, Cl'). The section 

 reminds one very much of a section of the adrenal. We have here a layer of lutein 

 cells well spread out, possibly due to the distention of its cavity by the ovum 

 and representing the corpus luteum, which, according to our conventions, is about 

 as old as this ovum appears to be. In other words, it is clear that the ovum devel- 

 oped within the cavity of the Graafian vesicle to which it belongs. The corpus 

 luteum, filled with blood, near the proximate pole of the ovary (figure 160), which 

 at first appears to represent the one from which the ovum came, is considerably 

 more advanced in development than the one containing the ovum; therefore it 

 belongs, in all probability, to a previous ovulation. Had it not been for the addi- 

 tional sets of sections we made, it would have been necessary to interpret this 

 specimen as Teacher, Bryce, and Kerr (1908) did theirs. The hemorrhage in the 

 ovary between the older corpus luteum and the cavity containing the ovum could 

 easily be viewed to indicate that the ovum invaded the ovarian tissue, as shown 

 by the illustrations of the authors mentioned. 



The older corpus luteum demonstrates once more very clearly that it is 

 imperative to standardize anew the development of the corpus luteum. It is 

 encircled by a very marked corpus fibrosum, which is wavy and forms a uniform 

 sheet about 0.5 mm. in thickness beyond the clot (figures 159 and 160). There 

 are few lutein cells within it. On the inner side of the corpus fibrosum there is a 

 thick layer of degenerated blood, and in the center a large mass of well-defined 

 red blood-corpuscles. Within the very center of the clot is a cleft which is curiously 

 lined and filled with red corpuscles, staining somewhat differently from those of 

 the rest of the clot. Also, at the periphery of this clot there is a curious vesicle 

 lying immediately under the outer fibrous layer, which may indicate a more 

 recent hemorrhage. At any rate, the lutein cells encircling the cavity containing 

 the clot and ovum prove quite conclusively that the ovum did not wander from 

 a distant Graafian vesicle and become implanted freely within the tissue of the 

 ovary. This conclusion has also been reached by Serebrenikowa (L912) in a report 

 of a case of ovarian pregnancy, which confirms fully that of van Tussenbroek. 



