62 



HISTOLOGIC PROPERTIES AND REACTIONS. 



uosa has a high reactivity with chloral hydrate and N. 

 sarniensis var. rose.a a low reactivity, so that N. elegans 

 takes after N. flexuosa in this reaction.) 



(2) N. crispa., in comparison with the other parent 

 N. elegans, shows higher reactions with polarization, 

 gentian violet, safranin, temperature, potassium iodide, 

 potassium sulphocyauate, calcium nitrate, uranium ni- 

 trate, and cupric chloride; lower reactions with iodine, 

 chloral hydrate, nitric acid, potassium sulphide, sodium 

 salicylate, and strontium nitrate; and the same or prac- 

 tically the same reactions with chromic acid, pyrogallic 

 acid, sulphuric acid, hydrochloric acid, potassium hydrox- 

 ide, sodium hydroxide, sodium sulphide, calcium nitrate, 

 cobalt nitrate, cupric chloride, barium chloride, and mer- 

 curic chloride. 



(3) The closeness of the curves of the two hybrids 

 is striking, the only important differences in their courses 

 being noted in the chromic-acid reactions, the reaction 

 of N. dainty maid being distinctly higher than in either 

 of the parents, and very much higher than in the other 

 hybrid N. queen of roses. The reaction of N. dainty 

 maid is closer to N. elegans, while that of N. queen of 

 roses is intermediate between the parents, but very much 

 closer to N. crispa. N. dainty maid shows higher reac- 

 tivities with polarization, iodine, calcium nitrate, and 

 copper nitrate; and lower reactivities with gentian violet, 

 safranin, temperature, chloral hydrate, and strontium ni- 

 trate; and the same or practically the same reactivities 

 with chromic acid, pyrogallic acid, nitric acid, sulphuric 

 acid, hydrochloric acid, potassium hydroxide, potassium 

 iodide, potassium sulphocyanate, potassium, sulphide, 

 sodium hydroxide, sodium sulphide, sodium salicylate, 

 uranium nitrate, cobalt nitrate, cupric chloride, barium 

 chloride, and mercuric chloride. 



(4) In N. crispa the very high reactions with polar- 

 ization, sulphuric acid, hydrochloric acid, potassium hy- 

 droxide, and sodium salicylate ; the high reactions with 

 nitric acid, potassium sulphide, and strontium nitrate ; 

 the moderate reactions with iodine, gentian violet, safra- 

 nin, temperature, and chromic acid; the low reactions 

 with chloral hydrate, and potassium sulphocyanate; and 

 the very low reactions with pyrogallic acid, potassium 

 iodide, sodium hydroxide, sodium sulphide, calcium ni- 

 trate, uranium nitrate, cobalt nitrate, copper nitrate, 

 cupric chloride, barium chloride, and mercuric chloride. 



(5) In N. elegans the very high reactions with polar- 

 ization, nitric acid, sulphuric acid, hydrochloric acid, 

 potassium hydroxide, sodium salicylate, and strontium 

 nitrate ; the high reactions with chloral hydrate, and 

 potassium sulphide ; the moderate reactions with iodine, 

 safranin, and chromic acid ; the low reactions with gen- 

 tian violet, temperature, and potassium sulphocyanate ; 

 and the very low reactions with pyrogallic acid, potas- 

 sium iodide, sodium hydroxide, sodium sulphide, calcium 

 nitrate, uranium nitrate, cobalt nitrate, copper nitrate, 

 cupric chloride, barium chloride, and mercuric chloride. 



(6) In the hybrid N. dainty mnid the very high reac- 

 tions with polarization, sulphuric acid, hydrochloric acid, 

 potassium hydroxide, and sodium salicylate; the high 

 reactions with iodine, nitric acid, potassium sulphide, and 

 strontium nitrate; the moderate reactions with safranin, 

 chromic acid, and potassium sulphocyanate; the low reac- 

 tions with gentian violet and temperature; and the very 



low reactions with pyrogallic acid, potassium iodide, 

 sodium hydroxide, sodium sulphide, calcium nitrate, 

 uranium nitrate, cobalt nitrate, copper nitrate, cupric 

 chloride, barium chloride, and mercuric chloride. 



(7) In the reactivities of the hybrid N. queen of 

 roses the very high reactions with chloral hydrate, sul- 

 phuric acid, hydrochloric acid, potassium hydroxide, so- 

 dium salicylate, and strontium nitrate; the high reactions 

 with polarization, nitric acid, and potassium sulphide; 

 the moderate reactions with iodine, gentian violet, safra- 

 nin, temperature, and chromic acid; the low reactions 

 with potassium sulphocyanate ; and the very low reactions 

 with pyrogallic acid, potassium iodide, sodium hydroxide, 

 sodium sulphide, calcium nitrate, uranium nitrate, cobalt 

 nitrate, copper nitrate, cupric chloride, barium chloride, 

 and mercuric chloride. 



The following is a summary of the reaction-intensi- 

 ties: 



11. COMPARISONS OF THE STARCHES OF NERINE 

 BOWDENI, N". SARNIENSIS VAR. CORUSCA MAJOR, 



1ST. GIANTESS, AND N. ABUNDANCE. 



In histologic characteristics, polariscopic figures, 

 reactions with selenite, qualitative reactions with iodine, 

 and qualitative reactions with the various chemical reag- 

 ents the starches of the parents exhibit properties in com- 

 mon, and also individualities by which they can be dif- 

 ferentiated. The starch of Nerine sarniensis var. corusca 

 major in comparison with that of N. bowdeni contains a 

 smaller number of compound grains and aggregates ; the 

 grains are more regular and less varied in form, and the 

 irregularities are due much more frequently to notches 

 and depressions at the margins ; and the flattened broad 

 forms are less flattened. The hilum is not so distinct, is 

 less frequently fissured, and is more eccentric. The 

 lamellre are not quite as distinct, they are more regular, 

 coarse lamella; are less numerous, the arrangements of 

 coarse and fine lamellfe differ from that which is observed 

 in N. bowdeni, and the number is somewhat less. In 

 size the grains are smaller, and there are not forms that 

 are as broad as are found in the other parent. In the 

 polariscopic, seleuite, and iodine reactions there are many 

 differences. In the qualitative reactions with chloral 

 hydrate, nitric acid, potassium iodide, potassium sul- 

 phide, potassium sulphocyanate, and sodium salicylate 

 there are also many differences, some of which are quite 

 interesting, and all are collectively of marked value in the 

 differentiation of the two starches. The starch of the 

 hybrid N. giantess, in comparison with the starches of 

 the parents, contains a much less number of compound 

 grains and aggregates than that of N. botvdeni, but 

 slightly more than in the starch of the other parent, and 

 the compound grains are partly of a type that is found 

 exclusively in N. bowdeni, and also partly of other types 



