74 



HISTOLOGIC PROPERTIES AND REACTIONS. 



barium chloride in which the reactions are almost abso- 

 lutely nil, yet even here there is at least the suggestion 

 of highest reactivity. The inclination to the properties 

 of the pollen parent are also strikingly manifested. 



COMPOSITE CURVES OF THE KEACTION-INTENSITIES. 



This section treats of the composite curves of the 

 reaction-intensities, showing the differentiation of the 

 starches of Narcissus iazetta grand monarque, N. poeti- 

 cus ornatus, and N. poetaz triumph. (Chart E 14.) 



The most conspicuous features of this chart are : 



(1) The close correspondence in the courses of all 

 three curves, and more particularly of the parental curves 

 which not only tend almost invariably to marked closeness 

 but also with few exceptions to keep below the hybrid 

 curve. 



(2) The curve of N. tazetla grand monarque tends 

 usually to be lower than the curve of the other parent. 

 It is distinctly lower in the reactions with chromic acid, 

 pyrogallic acid, nitric acid, and hydrochloric acid; 

 slightly lower or nearly the same with potassium hydrox- 

 ide, potassium sulphocyauate, potassium sulphide, so- 

 dium hydroxide, sodium sulphide, sodium salicylate, cal- 

 cium nitrate, uranium nitrate, strontium nitrate, cobalt 

 nitrate, copper nitrate, cupric chloride, barium chloride, 

 and mercuric chloride ; higher with iodine, gentian violet, 

 temperature, and chloral hydrate; and the same or prac- 

 tically the same with polarization, safranin, and sul- 

 phuric acid. 



(3) In N. tazetta grand monarque the very high re- 

 action with sulphuric acid ; the high reactions with 

 hydrochloric acid and sodium salicylate ; the moderate 

 reactions with polarization, iodine, gentian violet, sa- 

 franin, chromic acid, and potassium sulphocyanate ; the 

 low reactions with temperature, pyrogallic acid, potas- 

 sium iodide, sodium hydroxide, sodium sulphide, and 

 strontium nitrate ; and the very low reactions with 

 chloral hydrate, nitric acid, potassium hydroxide, potas- 

 sium sulphide, calcium nitrate, uranium nitrate, cobalt 

 nitrate, copper nitrate, cupric chloride, barium chloride, 

 and mercuric chloride. 



(4) In N. poeticus ornatus the very high reactions 

 with sulphuric acid and hydrochloric acid ; the high reac- 

 tions with chromic acid and sodium salicylate ; the moder- 

 ate reactions with polarization, safranin, and potassium 

 sulphocyanate; the low reactions with gentian violet, 

 temperature, pyrogallic acid, nitric acid, potassium hy- 

 droxide, potassium iodide, sodium hydroxide, sodium sul- 

 phide, calcium nitrate, strontium nitrate, and the very 

 low reactions with chloral hydrate, potassium sulphide, 

 uranium nitrate, cobalt nitrate, copper nitrate, cupric 

 chloride, barium chloride, and mercuric chloride. 



(5) In the hybrid the very high reactions with sul- 

 phuric acid, hydrochloric acid, and sodium salicylate ; the 

 high reactions with chromic acid and potassium sulpho- 

 cyanate ; the moderate reactions with polarization, iodine, 

 gentian violet, safranin, pyrogallic acid, potassium hy- 

 droxide, potassium iodide, and sodium hydroxide; the 

 low reactions with temperature, chloral hydrate, nitric 

 acid, sodium sulphide, calcium nitrate, and strontium 

 nitrate; and the very low reactions with potassium sul- 

 phide, uranium nitrate, cobalt nitrate, copper nitrate, 

 cupric chloride, barium chloride, and mercuric chloride. 

 The following is a summary of the reaction-intensities : 



15. COMPARISONS OF THE STARCHES OF NARCISSUS 

 GLORIA MUNDI, N. POETICUS OKNATUS, AND N. 

 FIERY CEOSS. 



In histologic characteristics, polariscopic figures, 

 reactions with seleiiite, reactions with iodine, and quali- 

 tative reactions with the various chemical reagents the 

 starches of the parents and hybrid possess properties 

 in common in varying degrees of development together 

 with occasional individualities which collectively in each 

 starch are distinctive. In histologic properties the 

 parental starches differ in both minor and major re- 

 spects. The starch of N. poeticus ornatus in comparison 

 with that of the other parents shows in th,e polarization 

 figure more distinctness and better definition, and other 

 differences; and with seleuite the quadrants are more 

 often well defined, less irregular in shape, the colors 

 not so often pure, and fewer grains have a greenish tinge. 

 In the qualitative iodine reactions no qualitative differ- 

 ences were recorded. In the qualitative reactions with 

 chloral hydrate, chromic acid, pyrogallic acid, nitric acid, 

 and sulphuric acid there are in each case characteristics 

 in common and also individualities. The starch of the 

 hybrid in comparison with the starches of the parents 

 shows a closer relationship to that of N. gloria mundi 

 in the form of the grains, character of the hilum, charac- 

 ter and arrangement of the lamella?, and in size ; but it 

 is closer to the other parent in the eccentricity of the 

 hilum. In the polarization figures and in the reactions 

 with selenite the relationship is closer to N. poeticus 

 ornatus. In the iodine qualitative reactions differences 

 between hybrid and parents, and between the latter were 

 noted. In the qualitative reactions with the chemical 

 reagents the hybrid shows certain resemblances to one 

 parent and others to the other, but it is, on the whole, 

 much more closely related to N. gloria mundi than 

 to N. poeticus ornatus. , 



Reaction-intensities Expressed by Light, Color, and Tempera- 

 ture Reactions. 

 Polarization: 



N. gloria mundi, low to very high, usually moderate to moderately 



high, value 60. 

 N. poeticus ornat., low to very high, lower than in N. gloria mundi, 



value 50. 

 N. fiery cross, low to very high, the same as in N. poeticus ornatua, 



value 50. 

 Iodine: 



N. gloria mundi, moderate, value 60. 



N. poeticua ornat, moderate, much less than in N. gloria muudi, 



value 40. 



N. fiery crosa, moderate, the same as N. gloria mundi, value 60. 

 Gentian violet: 



N. gloria mundi, light to moderate, value 40. 



N. poeticua ornat., light to moderate, much lesa than in N. poeticus 



mundi, value 30. 

 N. fiery cross, light to moderate, intermediate between the parents, 



value 35. 

 Safranin: 



N. gloria mundi, moderate, value 40. 



N. poeticus ornat., moderate, higher than in N. gloria mundi, 



value 45. 

 N. fiery cross, moderate, the same as in N. gloria mundi, value 40. 



