NARCISSUS. 



81 



to a moderate separation being in the reactions with 

 chromic acid and nitric acid. 



(2) In N. abscissus in comparison with the other 

 parent the higher reactions with polarization, chromic 

 acid, and nitric acid ; the lower reactions with iodine, 

 gentian violet, safranin, and temperature ; and the same 

 or practically the same reactions with chloral hydrate, 

 pyrogallic acid, and sulphuric acid. 



(3) In N. abscissus the very high reaction with sul- 

 phuric acid ; the high reaction with chromic acid ; the 

 moderate reactions with polarization, iodine, safranin. 

 and pyrogallic acid ; the low reactions with gentian violet, 

 temperature, and nitric acid; and the very low reaction 

 with chloral hydrate. 



(4) In N. poeticus poetarum the very high sulphuric- 

 acid reaction ; the absence of a high reaction ; the moder- 

 ate reactions with polarization, iodine, safranin, tem- 

 perature, and pyrogallic acid; the low reactions with 

 gentian violet, chromic acid, and nitric acid; and the 

 very low reaction with chloral hydrate. 



(5) In the hybrid the very high reaction with sul- 

 phuric acid ; the absence of a high reaction ; the moderate 

 reactions with polarization, iodine, safranin, chromic 

 acid, and nitric acid; the low reactions with gentian 

 violet, temperature, and pyrogallic acid; and the very 

 low reaction with chloral hydrate. 



The following is a summary of the reaction-intensi- 

 ties (10 reactions) : 



10. COMPARISONS OF THE STARCHES OF NARCISSUS 

 ALBICANS, N. ABSCISSUS, AND N". BICOLOR APRICOT. 



In histologic characteristics, polariscopic figures, 

 reactions with selenite, qualitative reactions with iodine, 

 and qualitative reactions with the various chemical reag- 

 ents the starches of the parents and hybrid exhibit prop- 

 erties in common in varying degrees of development 

 together with certain individualities which collectively 

 in each case are distinctive of the starch. In his- 

 tologic properties there are certain well-defined differ- 

 ences between the starches of the parent.s. In Narcissus 

 absrissits compared with the other parent the polari- 

 scopic figure is not so well defined, and there are minor 

 differences in the lines; and with selenite the quadrants 

 are not so clean-cut and are more irregular, the colors 

 are more often pure, and more grains have a greenish 

 tinge. In the iodine reactions no qualitative difference 

 was recorded. In the qualitative reactions with chloral 

 hydrate, chromic acid, pyrogallic acid, nitric acid, and 

 sulphuric acid there are both properties in common and 

 differences which are quite definite. The -starch of the 

 hybrid has fewer compound grains than in either parent, 

 and in form generally shows a closer relationship to 

 N. albirans than to N. abscissus. While the eccentricity 

 of the hilum is about the same in all three starches, the 

 character of the hilum is somewhat closer to that of 

 6 



N. abscissus. In the character of the lamellae and in the 

 size of the grains the relationship is closer to N. albicans. 

 In the character of the polariscopic figure and the appear- 

 ances with selenite the relationship is much closer to 

 N. albicans. In the qualitative iodine reactions the raw 

 grains show a closer relationship to N. albicans, but 

 after heating the relationship is closer to the other 

 parent. In the qualitative chemical reactions peculiari- 

 ties of both parents are observed. With chloral hydrate 

 the reactions, on the whole, more closely resemble those 

 of N. albicans; but in those with chromic acid, pyrogallic 

 acid, nitric acid, and sulphuric acid they resemble more 

 closely those of the other parent. There are also certain 

 individualities in the way of accentuation in the hybrid. 



Reaction-intensities Expressed by Light, Color, and Tempera- 

 ture Reactions. 

 Polarization: 



N. albicans, low to high, value 37. 



N. abscissus, low to high, higher than in N. albicans, value 43. 

 N. bicolor apricot, low to high, the same as in N. albicans, value 37. 

 Iodine: 



N. albicans, moderate, value 55. 



N. abscissus, light to moderate, much less than in N. albicans, 



value 40. 

 N. bicolor apricot, moderate, intermediate between the parents, 



but much closer to N. albicans, value 53. 

 Gentian violet: 



N. albicans, light to moderate, value 40. 



N. abscissus, light to moderate, lighter than in N. albicans, value 33. 



N. bicolor apricot, light to moderate, the same as N. albicans, 



value 40. 

 Safranin : 



N. albicans, moderate, value 50. 



N. abscissus, moderate, less than in N. albicans, value 47. 

 N. bicolor apricot, moderate, the same as N. albicans, value 50. 

 Temperature: 



N. albicans, in majority at 70.2 to 72, in all at 73 to 75, mean 74. 

 N. abscissus, in majority at 69.5 to 71, in all at 73 to 74, mean 



73.5. 



N. bicolor apricot, in majority at 71 to 72. 5, in all at 74 to 76, 

 mran 75. 



The reactivity of N. albicans is higher than that of 

 the other parent in the reactions with iodine, gentian 

 violet, and safranin ; and lower in those with polariza- 

 tion and temperature. The reactivity of the hybrid is the 

 same or practically the same as that of N. albicans with 

 polarization, gentian violet, and safranin; intermediate 



TABLE A 19. 



