64 PROCEEDINGS OF THE ACADEMY OF [Feb., 



when two of the lateral buds split open, the inner pair of green stipules 

 visible. March 25: A lower lateral bud with one leaf expanded, 

 another leaf was folded. March 28: Another lower lateral bud with 

 two leaves out, one only expanded. April 5: Terminal bud casting 

 off the outer brown stipules, one leaf unfolded. April 13. Twig dead. 



(B). — Twig dead, no response. 



(C). — March 11: Bud split open. March 15: First inner pair of 

 green stipules visible. March 18: Buds well opened, but first leaf 

 not unfolded. March 20: First leaf expanded. March 25: Terminal 

 and lateral buds each showing one green leaf. March 28: Leaves 

 flat, buds rapidly expanding. April 1 : Twig gone bad. 



Sulphuric Acid (five-drop solution). — No response. 



Sulphuric Acid (twenty-drop solution). — March 11 : Two upper buds 

 with outer stipular scales slightly eased apart. March 15: Scales more 

 eased apart. March 18: Buds burst. March 20, March 25, March 28, 

 April 1: No advance. April 13: Twig dead. 



Hydrochloric Acid (five drops). No response. 



Nitric Acid (five drops). — No response. 



Salix babylonica. — The chemicals used with the weeping willow 

 were: Chromic acid, one per cent.; two grams ammonium nitrate in 

 200 cubic centimeters of water; five decigrams of sodium bicarbonate 

 in fifty cubic centimeters of water; five decigrams of sodium chloride 

 dissolved in fifty cubic centimeters of water; ten drops of chemically 

 pure hydrochloric acid in fifty cubic centimeters of water; filtered 

 water; 100 cubic centimeters of ether mixed with 500 cubic centi- 

 meters of water; 200 cubic centimeters of chemically pure ammonium 

 hydrate in 1,000 cubic centimeters of water; picric acid; saturated 

 solution of corrosive sublimate. The results obtained in this series of 

 experiments possess considerable interest. 



Chromic acid, ammonium nitrate, sodium bicarbonate, sodium 

 chloride, ether water, ammonium hydrate, picric acid, corrosive sub- 

 limate gave no response. 



Filtered Water (A). — No response was shown until March 18 when 

 the buds of the female spike opened. March 20 : Roots well devel- 

 oped, pistillate spikes one inch long. April 1, April 5: Twig still 

 continued its growth. April 13: Twig withered. 



(B) First response on March 11 when secondary roots began to 

 form, no bud reaction. March 15: Pistillate spikes one inch long; 

 roots pink, three inches long, numerous. March 18: Leaf buds open, 

 first green leaves expanded. March 20: Pistillate spikes two inches 



