1909.] NATURAL SCIENCES OF PHILADELPHIA. 59 



were green. On March 8 no reaction was shown by the twigs in the 

 chromic acid, nitric acid, menthol water and one bottle of filtered water. 

 In fact the twigs in the nitric acid did not respond and were dead by 

 March 20. The buds, however, in the menthol water, chromic acid and 

 filtered water had responded on March 11. The subsequent history 

 of the twigs in the different solutions is as follows : 



Chromic Acid. — March 11: The buds of the two upper nodes were 

 developed. March 15: The two topmost buds were expanded, but 

 the leaves were still folded.. March 18: The leaves of the top pair of 

 buds were fully expanded, with the terminal bud one and a half inches 

 long. March 25: No advance. 



Picric Acid. — March 11: Upper buds were green. March 15: A 

 few end buds were green, but not opened. March 18: Upper buds 

 burst, leaves green. March 20: Leaves hardly unfolded. March 25: 

 No advance. March 28: Leaves hardly unfolded. March 25: No 

 advance. March 28: Twigs dead. 



Ammonium nitrate. — March 11 : All of the buds were burst and some 

 of the leaves were expanded. March 15 : Nearly all of the buds showed 

 leafy branches one and a half inches long, with two to three pairs of 

 leaves expanded. March 18: All of the green leaves were spotted and 

 unhealthy looking. March 20 : All of the expanded leaves were dead, 

 and on March 28 all of the twigs were dead. 



Sodium chloride. — March 1 1 : The three upper buds were enlarged 

 and ready to burst, March 15, March 18, March 20, March 25: No 

 advance in the development of these buds took place, and on March 

 28 the twigs w T ere dead. 



Hydrochloric Acid (five-drop solution). — March 11: The buds were 

 burst open, but the leaves were still folded together; only the lower- 

 most leaf was expanded. March 15: The upper two pairs of buds had 

 grown to a branch two inches long, with four pairs of opposite leaves 

 unfolded. March 18, March 20: All leaves of the branches expanded. 

 March 25, March 28, April 1 : No advance. April 5 : Twig dead. 



Menthol Water.— March 11: Middle buds burst. March 15: Three 

 buds were green, but not fully opened. March 18, March 25, March 28, 

 April 1 : No advance. April 5 : Twig dead. 



Filtered Water {A). — If the twig in this experiment is contrasted 

 with the twig in the other bottle of water, it is evident that its vitality 

 must have been impaired in some way before the experiments began, 

 hence the results obtained. On March 8 there was no response, as in 

 the other case March 11. The middle and lower buds had burst, the 

 leaves were flat but still erect. March 15: All of the buds had devel- 



