30 PROCEEDINGS OF THE ACADEMY OF [Jan., 



The Female. — It has already been shown that the female, in the 

 5- and 6-hour mice, is an oval cell with rather compact cytoplasm 

 and a vesicular nucleus with a large central body, the karyosome. 

 It is illustrated in figs. 33 to 37. The nuclear structure is com- 

 paratively simple and is portrayed well enough by the figures. The 

 nuclear membrane is usually clear cut, and there is always present 

 a large karyosome which takes the acid rather than the basic stain. 

 The karyosome frequently has associated with it one or more sharply 

 basophil granules, and may or may not show strands extending out 

 toward the nuclear membrane. 



It is difficult, however, to show the cytoplasm as it actually occurs, 

 and in the main this has been done in the figures in a purely conven- 

 tional maimer. Its fundamental structure appears to be alveolar 

 with the alveoles quite small and frequently tending to be variable 

 in size. The picture, however, is greatly confused by the fact that 

 in general both spongioplasm and hyaloplasm stain with much the 

 same degree of intensity and both have a rather high affinity for 

 plasma stains. Figs. 35, 77, 78, and 81 are attempts to show the 

 conditions as they actually occur. In fig. 35 (Plate II) the alveolar 

 structure is evident, although somewhat obscured, while it is seen 

 much more clearly in fig. 81 (Plate V) . Parasites in which the alveolar , 

 structure was as obvious as in fig. 81 could be found, but they were 

 rare. Figs. 34, 77, and 78 show the cytoplasm to consist of an aggrega- 

 tion of poorly defined rounded bodies lying in a paler matrix. This was 

 a frequent appearance. The black granules seen in some of the figures 

 represent the black granules usually to be seen in material stained 

 with iron hematoxylin. Frequently,' also, the cytoplasm is vacuolated. 



The general appearance here described does not undergo any great 

 change. Thus, figs. 28 (mouse 247) and 79 (mouse 113), respectively 

 4- and 9-hour stages, are much alike, as are figs. 77 and 78, respect- 

 ively, from 6- and 9-hour mice. 



There is, however, one phenomenon presented which appears to 

 he of interest. One phase of this is shown in figs. 81 and 82. In 

 each of these the nucleus presents somewhat the appearance of a 

 wheel. There is in each case a central acidophil karyosome which 

 si mis out strands to the periphery. Around the periphery are a 

 number of basophil granules. This condition was not at all infrequent 

 and appeared to be especially characteristic of the intermediate 

 periods of 9 or 10 hours. It was, however, seen in mouse 77, a 

 15-hour stage. We also have the condition illustrated in fig. 83, 

 where there is some basophil material outside of the nucleus. 



