NERINE. 



481 



of about HO per rent of the grains ami il'.i per rent of the 

 total slaivh in (Hi minutes. (Chart D 189.) The cleft 

 at the hilum and fissures proceeding therefrom react as 

 in the parents, but the enlargement and refractivity are 



more quickly lost. The proximal end of a number of 

 grains, as well as rare entire grains, resist gelal ini/ation, 

 but they are less resistive than in the paivnls. The reac- 

 tions are, on the whole, closer to those of C. nioorei. 



5. NERINE. 



This genus includes 9 or 10 species of South American 

 bulbous plants, the best known being N. sarnicnsis or the 

 Guernsey lily. A number of the members of the genus 

 have been known as belonging to other genera of Amaryl- 

 lidaceie, chiefly to Amaryllis. The starches from three 

 sets of parent-stocks and hybrid-stocks were studied : 



10. N. crispa Hort. (seed parent), N. elegans (N. flexuosa X N. 



santicnsis var. rosea) (pollen parent), N. dainty maid 

 (hybrid), and N. queen of roses (hybrid). 



11. A', bowdeni Hort. (seed parent), N. sarnicusis var. corusca 

 major Hort. (pollen parent), N. giantess (hybrid), and N. 

 abundance (hybrid). 



12. N. sarniensis var. corusca major Hort. (seed parent), N. 

 eurvifolia var. fothergilli major (X. fothergilli major 

 Hort.) (pollen parent), and JV. glory of sarnin (hybrid). 



All of the specimens were obtained from the growers, 

 Barr and Sons, London. 



10. STARCHES OF NERINE CRISPA, 1ST. ELEGANS, N". 



DAINTY MAID, AND 1ST. QUEEN OF ROSES. 



NERINE CRISPA (SEED PARENT). 



(Plate 6, figs. 31 and 34; Charts D 190 to D 210.) 

 HISTOLOGIC PEOPEBTIES. 



In form the grains are usually simple and isolated. 

 N"o aggregates are noted and a very few compound 

 grain of two types, of which the first has two hila set 

 close together at the proximal end of a large grain, 

 and the second of the two moderately large grains each 

 with from 4 to 8 lamella? surrounded by 1 or 2 common 

 secondary lamella?. The grains are usually moderately 

 regular in form and any irregularity is due to the fol- 

 lowing causes: (1) Secondary lamellae whose longitu- 

 dinal axis are at varying angles with the longitudinal 

 axis of the primary grains; (2) moderately large, 

 rounded projections from the proximal or distal ends 

 or sides; (3) rounded, shallow depressions in the margin 

 at various points; (4) a small rather deep notch at the 

 distal end; (5) a greater development of one part of 

 the distal end or of one side than of the rest; (6) devia- 

 tion of the longitudinal axis with a consequent curvature 

 at the distal end or in the middle. The conspicuous 

 forms are ovoid, usually rather broad, but sometimes 

 slender; elongated elliptical with flattened distal end; 

 and, among the small grains, round and nearly round. 

 There are also plano-convex, triangular with rounded 

 angles, modified pyriform, napiform, and spatulate. 

 The proximal end of most of the forms is the larger and 

 more rounded, and in only a few is the reverse the case. 



The hilum when not fissured is a moderately distinct 

 round spot. It is fissured in about 75 per cent of the 

 grains and the fissures take the following forms: (1) 

 A single, small, straight, transverse, or oblique line 

 which is sometimes much branched; (2) small or large 

 flying-bird; (3) T- or Y-shaped placed in the longitu- 

 dinal and transverse axes or obliquely. In the compound 

 grains the hila are sometimes separated by a cleft and 

 may also have a common fissure remaining horizontally 



through both. The hilum is eccentric from 0.4 to 0.2, 

 usually, 0.26 of the longitudinal axis. 



Thelain/'l/n arc very distinct, usually rather fine lines, 

 which when near the hiluni are circular and continuous, 

 but become discontinuous near the distal end and have 

 the form of the outline of the grain, the form being some- 

 what modified in certain cases. In a largo number of 

 grains the lamella? are fine near the distal end and not 

 so fine near the hilum, in others they are of equal size 

 in almost all parts of the grain, and in still others they 

 are finer near the hilum and not so fine near the distal 

 margin. There is characteristically one very distinct, 

 coarse, continuous lamella which may be near the hilum, 

 or, rarely, near the margin, but is usually about midway 

 between the hilum and the margin. The number counted 

 on the larger grains varies from 20 to 40, usually 34. 



The size of the grains varies from the smaller which 

 are 6 by Gp. to the larger elongated f onus which are 50 

 by 26fi, rarely 56 by 30/t, and the larger broader forms 

 which are 50 by 3(>//, in length and breadth. The common 

 sizes are 34 by 22/j. and 34 by 28/t, respectively. 



POLABISCOPIC PROPERTIES. 



The figure is slightly to very eccentric, distinct, and 

 usually clear-cut. The lines are usually fine and intersect 

 obliquely, but in a moderate number of grains they inter- 

 sect at right angles. They are sometimes straight but 

 equally as often bent, and occasionally are bisected. 

 Double figures are not very numerous but do occur. 



The degree of polarization is moderate to very high 

 (value 85). It varies in different grains, a moderate 

 number having a moderately high, a few having a very 

 high, and the majority having a high polarization. Occa- 

 sionally moderate variation in polarization in the same 

 aspect of a given grain is present. 



With selenite the quadrants are usually well defined, 

 often somewhat irregular in shape, and always unequal 

 in size. The colors are usually pure, the yellow less often 

 pure than the blue. A few of the grains have a greenish 

 tinge. 



IODINE REACTIONS. 



With 0.25 per cent Lugol's solution the grains color a 

 moderate blue with a slight tinge of violet (value 45) 

 which very rapidly becomes deep to very deep, the bluish 

 tint increasing. With 0.125 per cent Lugol's solution 

 the grains color a moderate to light blue with a slight 

 tinge of violet, which deepens rapidly, becoming more 

 blue, the depth varying from moderately deep in most 

 grains to very deep in a few small grains. After heating 

 in water until the grains are gelatinized, and then adding 

 a 2 per cent Lugol's solution, the grains color a moder- 

 ately deep to very deep indigo-blue, the capsules usually 

 can not be distinguished, but if so are colored violet; 

 the solution colors a moderately deep indigo-blue. If the 

 preparation is boiled for 2 minutes, and then treated 

 with an excess of 2 per cent Lugol's solution, the grain- 

 residues color light to deep indigo-blue; the capsules, of 



