NERINE. 



491 



NERINE QUEEN OF ROSES (HYBRID). 



(Plate 6, fig. 36; Charts D 190 to D 210.) 

 HISTOLOGIC PROPERTIES. 



In form the grains are usually simple and isolated, 

 but there arc more aggregates containing more component 

 grains, and more compound grains, than in either parent 

 or in N. dainty maid. The compound grains are of the 

 character described under N. elegans. The grains are 

 less regular than those of N. clegans, but more regular 

 than those of N. crispa and N. dainty maid. The causes 

 of any irregularities are the same as in N. elegans, ex- 

 cept that notches in the distal margin are not so common 

 as in that starch. The conspicuous forms are ovoid and 

 elliptical with flattened distal end, even among the small 

 grains, in which respect it resembles N. elegans; however, 

 there are more small, round grains than in that starch. 

 There are also pyriform, triangular with rounded angles, 

 and a few of the spatulate forms seen in 2V. crispa. 



The grains of this hybrid are more like 2V. elegans 

 than TV. crispa in form. This hybrid differs from 2V. 

 dainty maid, in a greater resemblance to 2V. crispa in 

 being more regular in form, and in having more aggre- 

 gates and compound grains. 



The hilum is as distinct as in 2V. crispa and 2V. dainty 

 maid, but is very rarely fissured, and in the latter respect 

 is more like 2V. elegans and 2V. dainty maid. The hilum 

 is eccentric from 0.36 to 0.17, usually 0.21 of the longi- 

 tudinal axis, more than in either parent. The character 

 and the eccentricity of the hilum are, on the whole, more 

 like 2V. elegans than 2V. crispa. 



2V. queen of roses and 2V. dainty maid more closely 

 resemble one another in the character of the hilum than 

 either resembles either parent, but both are nearer 2V. 

 elegans than 2V. crispa. 



The lamella are about as fine and as distinct and are 

 of the same character and arrangement as in 2V. crispa; 

 the number counted on the larger grains varies from 16 

 to 30, usually 24 ; in character and arrangement they are 

 closer to 2V. crispa than to 2V. elegans, but in number 

 closer to 2V. elegans. The lamellae of 2V. queen of roses 

 are nearer, on the whole, to 2V. crispa than are those of 

 2V. dainty maid, and the latter are nearer to 2V. elegans 

 than to 2V. crispa. 



The size of the grains varies from 3 by 3/j. for the 

 smaller to 44 by 28/j, in length and breadth for the larger. 

 The common size is 22 by IG/j.. In size 2V. queen of roses 

 is smaller than either parent, but nearer 2V. elegans than 

 2V. crispa. 2V. queen of roses is smaller than 2V. dainty 

 maid, but slightly closer to 2V. dainty maid than the 

 latter is to either parent, but not so close to 2V. elegans 

 as is 2V. dain ty maid. 



POLARISCOPIC PROPERTIES. 



The figure is slightly to very eccentric, usually eccen- 

 tric, distinct, and clear-cut as in 2V. elegans. The lines 

 are commonly finer than in 2V. elegans but intersect 

 obliquely, and are bent and bisected as in 2V. elegans. 



The degree of polarization is moderate to very high 

 (value 77), less than in either parent or in 2V. dainty 

 maid, and there are more of the moderately high than in 

 either parent or in 2V. dainty maid. 



With selenite the quadrants are usually well defined, 

 and often somewhat more irregular in shape than in 2V. 



elegans and always unequal in size. The colors are less 

 often pure than in 2V. elegans, and the yellow is less often 

 pure than the blue. A very few of the grains have a 

 greenish tinge. 



In degree of polarization, character of the figure, and 

 appearances with selenite 2V. queen of roses is closer to 

 2V. elegans than to 2V. crispa, and closer to 2V. elegans 

 in all polariscopic characteristics than in 2V. dainty maid. 



IODINE REACTIONS. 



With 0.25 Lugol's solution the grains color a mod- 

 erate' blue witli a tinge of violet (value 55), the same 

 as in 2V. elegans, but deeper than in 2V. crispa and not 

 so deep as in 2V. dainty maid, and the color rapidly 

 deepens to very deep as in 2V. elegans. With 0.125 per 

 cent Lugol's solution, the grains color a moderate to 

 light blue with a tinge of violet, the same as in 2V. elegans 

 and the color deepens, as in those grains, to a moderately 

 deep to deep. After heating in water until the grains 

 are gelatinized, and then adding 2 per cent Lugol's 

 solution, the grains color a moderately deep to very deep 

 indigo-blue, as in 2V. crispa; the capsules usually can not 

 be distinguished, but when they can they color a purer 

 violet as in 2V. crispa; the solution colors a moderately 

 deep indigo-blue as in 2V. crispa. If the preparation is 

 boiled for 2 minutes, and then treated with an excess of 

 2 per cent Lugol's solution, the grain-residues color light 

 to deep indigo-blue, as in 2V. crispa, the capsules (not all 

 of which can be distinguished) a pure violet as in 2V. 

 crispa, and the solution a deep indigo-blue. 



Quantitatively and qualitatively the iodine reactions 

 of the unheated grains of 2V. queen of roses are closer to 

 2V. elegans, and qualitatively the reaction of the gelati- 

 nized grains are closer to 2V. crispa. 



The iodine reactions of the unheated grains of 2V. 

 queen of roses are closer to 2V. elegans than are those 

 of 2V. dainty maid; and those of the gelatinized grains 

 more closely resemble 2V. crispa, while those of 2V. dainty 

 maid resemble 2V. elegans. 



ANILINE REACTIONS. 



With gentian violet the grains color very lightly at 

 once, and in half an hour they are light to moderately 

 colored (value 40), the same as in 2V. crispa. As in the 

 parents there is inequality of coloring between different 

 grains and in individual grains. 



With safranin the grains color very lightly at once, 

 and in half an hour they are moderately colored (value 

 50), the same as in 2V. crispa. As in the parents there 

 is inequality of coloring between different grains and in 

 individual grains. 



In the reactions to aniline stains, 2V. queen of roses 

 shows a closer relationship to 2V. crispa than to 2V. ele- 

 gans; it is closer to 2V. crispa than to 2V. dainty maid, 

 which in turn is closer to 2V. elegans. 



TEMPERATURE REACTIONS. 



The majority of the grains are gelatinized at 68 

 to 69.1 C., and all at 71 to 72.8 C., mean 71.9 C., 

 or 1.2 higher than in 2V. crispa, 4 lower than in 2V. 

 elegans, and 1.3 C. lower than in 2V. dainty maid. 



'The temperature of gelatinization of 2V. queen of roses 

 is intermediate between the parents and closer to 2V. 

 crispa, than to 2V. clegans, and it is closer to 2V. crispa 

 than is that of N. dainty maid. 



