The Chemistry of Light-Production in Luminous Organisms. 



217 



flask be oxygenated by shaking, before judging as to the emission or 

 absence of light. Observations were made after 10 minutes, 1 hour, 

 and 24 hours. 



TABLE 15. Effect of dilution of sea-water ivith water and with m cane-sugar solution. 



LUMINOUS BACTERIA AND BALANCED SOLUTIONS. 



Not only must luminous bacteria be bathed with a certain concen- 

 tration of salts, but any salt of sea-water is not sufficient. It must be 

 a particular salt, although luminous bacteria, as indicated in table 16, 



TABLE 16. Effect of various combinations of the salts of sea-water. 



are not so affected by the pure monovalent cations of sea-water as 

 are most cells. 



Note the independence of these bacteria of a balanced medium. 

 The bacteria live and phosphoresce in pure NaCl without the addition 



