250 



Papers from the Department of Marine Biology. 



subjected to m/8 butyl alcohol for an hour in a tube containing sand. 

 Some were then returned to sea-water and recovered, showing that the 

 animals were truly narcotized. The remaining animals were shaken 

 up and broken to pieces by the sand. At the same tune, a control lot 

 kept for the same period in sea-water in a tube containing sand were 

 shaken and broken up. A comparison of the two tubes showed that 

 the anesthetized animals were not nearly so bright as the control lot. 

 A microscopic examination of the material showed that the cells were 

 completely broken to pieces into irregular fragments by the sand. A 



TABLE 6. Effect oj anesthetics. 



Solution in sea-water. 



Chloroform: 



Saturated . . . . 

 1/2 saturated . 

 1/3 saturated . 



1/4 to 1/8 saturated . 



Ether: 

 m/2. 



m/4. 



m/8.. 

 m/16. 

 m. . 



Ethyl alcohol: 



2m 



1.5m 



m 



m/2 to m/4 . 



Butyl alcohol: 



m/2 



m/4 



m/8 



m/16 to m/32 . 



Chi ore tone: 



Saturated 



1/2 saturated 



1/4 to 1/8 saturated. . . 

 1/16 to 1/32 saturated 



Thymol: 



Saturated to 1/4 satu- 

 rated. 



1/S saturated 



1/16 saturated 



1/32 saturated 



1/01 to 1/128 saturated 



Luminous response. 



Constant glow 1 to 2 minutes. 



Constant glow 4 to 8 minutes. 



Constant glow 2 hours, then normal re- 

 sponse, owing to evaporation of chloro- 

 form. 



Normal response > 12 hours (exp. discon- 

 tinued). 



Constant glow 4 minutes 



Constant glow 30 minutes; becomes 

 brighter, then fainter. 



Constant glow 90 minutes 



Normal response > 1 day (exp. discon- 

 tinued). 



Constant glow 1 minute 



Constant glow 5 minutes 



Constant glow 30 minutes 



Normal response >14 hours (exp. discon- 

 tinued). 



Constant glow 1 minute 



Constant glow 5 minutes 



Constant glow 1 5 hours (faint) 



Normal response > 14 hours (exp. discon- 

 tinued). 



No response 



Constant glow 4 minutes 



Constant glow 15 minutes (faint) 



Normal response > 24 hours (exp. discon- 

 tinued). 



Constant glow 1 j to 2 minutes 



Constant glow 6 minutes 



Constant, glow <1 hour, normal response 

 4. minutes at start (poor). 1 



Normal response < Is hours (poor) 



Normal response >o days (exp. discon- 

 tinued. 



Duration of tentacle 

 movement. 



30 minutes. 



<5 minutes. 

 <5 minutes. 



<30 minutes. 



<5 minutes. 

 < 10 minutes. 



<'_'() minutes. 



Stops immediat ely 

 < 10 minutes. 



< 1.1 minutes 



'The normal response at the start is doubtless due to the delay in taking effect on certain of 

 the animals, 



