APPENDIX I. 267 



as to the accuracy of the results obtained. The investigation may require a 

 considerable proportion of the time for a number of years. 



In thus preparing this elaborate program, there is not the slightest desire 

 to preempt any portion of the field, for, as Professor Lusk recently said: "The 

 importance of the problem is too great not to have the work repeated in as 

 large a measure as possible in at least two different laboratories." 



I shall appreciate most fully any adverse criticisms that you may see fit to 

 make of this program. Any additions to it will be most gratefully received, 

 and obviously full credit will be given for such suggestions. 



Will you not kindly send to this laboratory copies of such reprints as you 

 have available bearing in any way upon the subject here outlined. Such 

 reprints will materially lighten our work and insure a correct and adequate 

 consideration of your own researches. 



The investigation will be undertaken primarily to establish the important 

 physiological relationships existing between the ingestion of alcohol and the 

 metabolism and the activities of the body functions. 



As an important correlative investigation, it is planned to carry out simul- 

 taneously an investigation on the psychological effects of alcohol, employing 

 the technique that will make the results as objective as possible. 



The program for the psychological study to accompany this research has 

 been prepared by Professor Raymond Dodge, the experimental psychologist 

 of the Nutrition Laboratory. 



FRANCIS G. BENEDICT. 



PHYSIOLOGICAL PROGRAM. 



I. Subjects (numerous in each class): 



1. Non-users of alcohol. 



2. Moderate occasional users. 



3. Habitual drinkers (exceeding 30 c.c. absolute alcohol per day). 



4. Excessive drinkers (with whom the effects of abstinence should be likewise studied). 



II. Alcohol doses. Controls if possible under conditions in which the subject will not know 



irhen alcohol is administered! 



1 . Ethyl alcohol in various forms. 



Pure alcohol, distilled spirits, wines, champagne, beers, ales, and hard cider should 



be used. 



The variation in effects of the different kinds of liquors, if any, to be determined on one 

 or two simple physiological or metabolic processes. If the effects in the 

 above are not found directly proportional to the amount of absolute alco- 

 hol present, this fact should be elaborated in a subsequent research, and 

 this present investigation should adhere to pure ethyl alcohol + water. 



2. Doses, amounts. 



a. One single dose, varying amounts. 



b. Repeated doses at varying intervals. 



3. How administered. 



a. By mouth (drinking). 



6. By mouth (stomach tube). 



c. Rectal enema. 



d. Inhalation of alcohol vapor. (Leonard Hill.) 



e. By the skin. 



Immerse hand or arm in vessel (arm plethysmograph) containing moderately 

 dilute alcohol. Is there any cutaneous absorption? 



Perhaps stimulate cutaneous circulation by massage or electricity and note 

 alcohol absorption. 



4. When administered. 



a. Empty stomach (cocktail) between meals drinking. 



b. With food. 



1. With protein. 



2. With fats. 



