46 ESTABLISHMENT OF VARIETIES IN COLEUS 



more than twice as many bud variations. Clone 13, which was derived 

 from a branch that was green-red blotched, gave a progeny (of several pat- 

 terns) of which 54 per cent were constant, but bud variations were very 

 infrequent. Clone 14, with nearly the same percentage of constant 

 plants, produced four times as many bud variations. This summary 

 of the data by clones irrespective of patterns shows a general irregular- 

 ity and lack of correlation between fluctuating variations and bud vari- 

 ations. The special interest, however, pertains to the clones 11 and 12, 

 which show that two branches apparently identical may have quite 

 different potentialities for constancy and for bud variations. 



Even more marked differences than these developed among the 

 various subclones. A study of pedigrees shows that in all patterns 

 and in all main clones there were certain lines of progeny much more 

 constant than many others. These could not be detected by any other 

 than a pedigree method. 



Clone 111 can be given as one of the most constant clones. Its 

 members numbered 34. Four cases of bud variation appeared; 3 were 

 a loss of yellow and 1 was a reversal of the position of the green and 

 yellow occurring in one-half of a leaf only. These 4 plants were other- 

 wise constant. Only 1 plant showed fluctuating variability, becoming 

 quite uniform for green-yellow spotted-red blotched. All the bud varia- 

 tions involved changes in the green and yellow. There were no marked 

 changes in the amount and distribution of epidermal red. As shown in 

 table 12, the percentage of constant plants was 85 and the ratio of bud 

 variations was 1 to 1,700. The clone was highly constant both in regard 

 to fluctuations and bud variations. 



On the other hand, the series of plants derived from plant 117 was 

 26 per cent lower in number of constant plants and gave nearly three 

 times as many bud variations, yet plants 111 and 117 were both 

 uniform and constant for the pattern green-yellow-red blotched and were 

 apparently identical. Until the autumn of 1912, plant 1171 was the 

 only one of the 17 plants grown in clone 11 that showed variation. It 

 gave during the summer, by sectorial variation in the main axis, 6 

 branches with the position of the green and the yellow reversed. The 

 plant was grown in a large pot during the winter and then grown out 

 of doors during the following summer. In the second summer two 

 more bud variations appeared on the part with green-yellow-red blotched 

 foliage, but on branches quite separated. Both were sectorial; one was a 

 loss of green, giving the yellow-red blotched pattern, and one was a loss 

 of yellow, giving the green-red blotched pattern. The plant possessed for 

 some time four patterns, each uniform for a certain part of the plant. 



The record of pedigree for the progeny of the plant can be given as 

 illustrating a clone in which bud variations occurred with a high ratio 

 of frequency. In table 13 the plants are arranged in generations 

 according to number. To trace the progeny or the ancestry of any 



