INGESTION OF PROTEIN DIETS. 293 



ments with two subjects. The observations were made in 2-hour 

 periods. The basal values were all determined on a different day from 

 that on which the metabolism after gluten was determined. The 

 amount of gluten bread taken in the last experiment by the second 

 subject, H. C. K., was considerably smaller than the amounts eaten 

 by the subject H. R. D.; H. C. K. also found it necessary to take a 

 greater quantity of skim milk with the bread. The proportion of nitro- 

 gen from the skim milk was therefore increased, but the experiment is 

 included in this section for additional information as to the metabolism 

 after the ingestion of gluten bread. Statistical data not included in 

 the tables or in the discussion of the experiments are given here: 



H. R. D., 9 h 01 m a. m. to 5 h 01 m p. in., May 2, 1906. 58.5 kilograms. 

 Urinated at 7 h 05 m a. m. and at beginning of every period. Quiet for most 

 part during experiment; drowsy between 9 a. m. and 10 a. m.; especially quiet 

 about 11.30 a. m.; read about three-fourths of time. Body-temperature: 

 36.71, 36.70, 36.71, 36.76, 36.73 C. Pulse rate, 67; respiration rate, 19. 



H. R. D., 9 h 30 m a. m. to 9*30 m p. m., May 9, 1906. 58.4 kilograms. 

 Took enema and urinated about 7 h 30 m a. m. and urinated at beginning of 

 every period. Distressed by rectal thermometer owing to temporary tender- 

 ness in lower part of rectum; removed thermometer at 10 h 31 m a. m. and ex- 

 changed it for another, telephoning once and opening food aperture twice for 

 the purpose; rest of time quiet and reading through first and second periods. 

 Sat idle from I h 30 m p. m. to 2 h 04 m p. m., slept from 2 h 04 ra p. m. to 2 h 32 m p. m., 

 read from 2 h 34 m p. m. to 9 h 30 m p. m.; drowsy at times during day. Body- 

 temperature: no record at beginning of experiment; subsequent records, 

 36.85, 37, 36.95, 36.95, 37.02, 36.78 C. Pulse rate, 63 ; respiration rate, 19. 



H. R. D., 9*10 m a. m. to 9 h 10 m p. m., May 17, 1906. 59.1 kilograms. 

 Urinated 7 h 15 m a. m. (after enema) and also at beginning of every period. Sat 

 quietly reading almost whole experiment. Drank 90 grams water at Il h 10 m 

 a. m. Body-temperature: 36.64, 36.62, 36.71, 36.53, 36.59, 36.60 C. 

 Pulse rate, 69; respiration rate, 20. 



H. C. K., 9 h 10 m a. m. to 5 h 10 m p. m., May 7, 1906. 74.4 kilograms. 

 Urinated and defecated about 7 h 10 m a. m. and urinated at beginning of every 

 period. Food difficult to swallow. Subject very quiet throughout experi- 

 ment; fell asleep twice in second period, but not drowsy rest of time; read 

 during most of experiment. Body-temperature : 36.34, 36.38, 36.53, 36.60, 

 36.66 C. ; no record at end of experiment. Pulse rate, 50; respiration rate, 19. 



DISCUSSION OF EXPERIMENTS. 



H. R. D., May 2, 1906. In the first experiment with this subject 

 100 grams of gluten bread were taken and 221 grams of skim milk. 

 The nitrogen content of the diet was 15.43 grams, the greater part of 

 this being contained in the gluten bread. During the 8 hours of the 

 experiment, the results of which are given in table 224, there was a 

 continuously increasing rise in the nitrogen excretion, also an incre- 

 ment in all of the factors of the metabolism. The maximum carbon- 

 dioxide production was in the third period, as was also the maximum 

 heat production. It was necessary for experimental reasons to com- 



