abstracts: bacteriology 337 



previously reported from Ireland) ; Leptometra celtica (Barrett and Mc- 

 Andrew); Hathrometra prolixa (Sladen) (Faeroe Channel); Trichometra 

 hibernica, sp. nov. (related to a species occurring off Newfoundland); 

 Trichometra delicata (a species described in 1911 from the Bay of Bis- 

 cay); Pentametrocrinus atlanticus (not hitherto known north of the 

 Bay of Biscay) Atelecrinus helgae, sp. nov. (related to the Caribbean 

 A. balanoides, and the first species of the genus to be discovered in 

 the east Atlantic) ; and Rhizocrinus lofotensis. A. H. C. 



BACTERIOLOGY. — -Further attempts to transmit poliomyelitis thru the 

 agency of the stable fly (Stomoxys calcitrans) . John F. Anderson, 

 Surgeon, U. S. Public Health Service, Director Hygienic Laboratory, 

 and Wade H. Frost, Passed Assistant Surgeon, U. S. Public Health 

 Service. Public Health Reports, 1913. 

 In an experiment conducted in October, 1912, we were able to infect 

 monkeys with poliomyelitis by exposing them to the bites of several 

 hundred Stomoxys, which were allowed, at the same time, to feed on 

 monkeys inoculated intracerebrally with poliomyelitis virus. From the 

 date of that experiment up to February, 1913, we have carried out a 

 number of additional experiments designed to throw light upon the 

 conditions necessary for the experimental transmission of poliomyelitis 

 thru Stomoxys calcitrans. The flies used in our later experiments have 

 been, in part, captured in the vicinity of the Hygienic Laboratory and, 

 in part, bred in the laboratory. In a considerable number of our experi- 

 ments we have undertaken to simulate summer conditions by maintain- 

 ing a temperature of 75° to 85° F. 



In these experiments monkeys inoculated with poliomyelitis have been 

 exposed to large number of Stomoxys at various stages of their incubation 

 period and during the period of developed sjanptoms. Fresh monkeys 

 have been exposed to these same flies simultaneously with the inoculated 

 monkeys and at various intervals thereafter. Stomoxys fed on defibri- 

 nated blood drawn from inoculated monkeys in all stages of the incu- 

 bation period of the disease and others fed on mixtures of normal mon- 

 key blood with poliomyelitis virus, have been allowed to feed on fresh 

 monkeys. In one experiment we inoculated a yearling colt intrave- 

 nously and intracerebrally with a large amount of poliomyelitis virus 

 and exposed this animal at once to some 2000 Stomoxys, which were 

 then allowed to bite fresh monkeys. Neither the colt nor the monkeys 

 showed symptoms in any way suggestive of poliomyelitis. 



Two experiments were carried out with flies caught in the immedi- 



