Life-history of the Meningococcus. 



373 



glucose. In the remaining two cnltures no acid was produced in 

 glucose. 



In every case incubation at 37'C. was continued for eight days. 



In the case of these four cultures the dilutions were made from 

 undiluted meningococcal serum supplied by the Lister Institute. 



Titre, 1/250 ; No. CI. ; date of tubing, 9/^/16 ; dates of agglutina- 

 tion. 8/3/16, 4/3/16, 29/4/lC, 29/4/16. 



Incubation at 37° C. was allowed for twenty-four hours before read- 

 ing, plus one hour at room temperature. 



C = complete agglutination. 



P = partial „ 



The agglutination tests of the remaining ten cultures were kindly 

 carried out by Dr. Lawes, using Gordon's method for identification of 

 " strains." 



The agglutination tubes and their contents were incubated at 56° C. 

 for twenty-four hours before being read. 



Cultures 8 and 9, 11 and 12, 13 and 14, were from three cases ; 

 cultures 1 to 7, as well as culture 10, being from eight separate cases ; 

 a, b, c, represent respectively dilutions 1/100, 1/200, 1/400. 



In summing up this demonstration of the morphology of the 

 meningococcus and of the so-called giant forms, I suggest the 

 following order of events, by no means dogmatically or finally, as 

 the subject is one of the greatest difficulty. Starting from one of 



"whatever its true botanical nature, whether 



the giant forms 



