512 



SUMMARY OF CUIUIBNT RESEA.11CHES RELATING TO 



agar is used instead of broth for tlie culture medium in the large tube. 

 This is melted, and before solidifying is mixed in the manner above 

 described with the emulsion. The agar is then sloped or distributed 

 over the walls of the tube by gentle rotation. Anaerobic cultures can 

 be made from a vein by substituting a hypodermic Sneedle for the 

 pipette. 



Fig. 1. 



Cultivation of the Parasite of Epizootic Lymphangitis.* — 

 A. Boquet and L. Negre state that the most favourable medium for the 

 cultivation of the above organism consists of a filtered decoction of 

 dried horse-dung in water (200 grm. to the litre) containing 1 p.c. 

 pepton, 1 • 8 p.c. agar, and 4 p.c. glucose. When pus, taken from an 

 unopened abscess, is sown on this medium and incubated at 24° C. to 

 26° C. the cryptococci are found, after eighteen to twenty-four hours, to 

 increase in size and to become rounded and granular. After forty-eight 

 hours the rounded forms are filled with oily drops and throw out 

 filaments which become segmented and attain a length of from 75 to 

 100 /A. Sub-cultures do not grow on this medium, however, but if the 

 surface of the horse-dung agar is smeared with pus a few small colonies 



* Bull. Soc. Path. Exot.,x. (1917) pp. 274-6. 



