ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 



557 



B. Technique.* 

 11) Collecting: Objects, including- Culture Processes. 



Apparatus for Decanting off Culture Fluids.f — W. Behrens 

 describes an apparatus constructed by V. Basila which is intended for 

 removing from a stock vessel definite quantities of culture media without 

 fear of contamination. 



The apparatus, made entirely of glass, consists of an Erlenmeyer's 

 flask A in connection with which is a spherical vessel D, the latter 

 having a suction-tube C and a discharge pipe B. By means of the taps 

 E and F, D can be shut off above and below. By means of the tap E 

 the communication between D and A and B can not only be cut off but 

 can be made with one or both simultaneously. The tap F is so bored 

 that it allows air to pass either way. 



Fig. 137. 



When the apparatus is to be used, cotton-wool is stuffed into the 

 bulb H, the flask is filled with culture fluid, and the whole having been 

 steam sterilised the caoutchouc plug is inserted. The tap E is turned 



* This subdivision contains (1) Collecting Objects, including Culture Pro- 

 cesses; (2) Preparing Objects ; (3) Cutting, including Imbeddiug and Microtomes; 

 (4) Staining and Injecting ; (5) Mounting, including slides, preservative fluids, &c. ; 

 («) Miscellaneous. t Zeitschr. wiss. Mikr., xix. (1903) pp. 429-31 (1 fig.). 



Aug. 19th, 1903 2 o 



