ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 767 



B. Technique. * 

 (1) Collecting: Objects, including- Culture Processes. 



Method of Preparing Sugar-free Bouillon.!— F. E. Montgomery 

 lias found that if meat infusion be sterilised previous to the inoculation 

 with the colon bacillus, an odorless sugar-free broth is obtained. The 

 method is as follows : To a portion of the fat-free beef, ground fine, add 

 double its weight of cold water, and bring slowly to a temperature of 50° 

 over water bath. Keep at this temperature for three hours, then strain 

 through muslin. Steam sterilise the infusion for three-quarters of an 

 hour. Allow to stand overnight in an ice-box, and then inoculate with 

 B. coll communis. Incubate at 37*5° for 18 to 24 hours, then boil and 

 filter. Next add ^ p.c. NaCl and £ p.c. peptone, and boil for three- 

 quarters of an hour. Neutralise with NaHO, filter and sterilise. 



Cultivation Medium for Algas.J — Gr. T. Moore finds that for general 

 purposes a modification of Beijerinck's medium is very satisfactory.. 

 This consists of ammonium nitrate, 0*5 grm. ; potassium phosphate, 

 0*2 grm. ; magnesium sulphate, 0'2 grm. ; calcium chloride, O'l grm. ;■ 

 distilled water, 1000 c.cm. ; iron sulphate, a trace. 



For blue-green Algge, the amount of ammonium nitrate should be 

 doubled, and the addition of from 1-2 p.c. glucose is often of benefit. 

 This solution may be used with silica jelly, though i-1 p.c. of agar 

 hardens it sufficiently for general purposes. 



Demonstration of Tubercle Bacilli in Sputum. § — A. Nebel advises 

 that the sputum be well shaken up with 8-10 times its volume of lime- 

 water. This renders it apparently homogeneous, and it is then centrifuged 

 for 2 minutes. The supernatant fluid is passed through a Berksfeld 

 filter ; and the deposit remaining on the filter removed, mixed with a drop 

 of water, and examined in the usual way. 



The author found that after centrifuging, the. sediment did not con- 

 tain more bacilli than fell to its lot on account of its weight and 

 volume. 



New Method of Isolating B. icteroides.||— J. Bandi makes use of 

 the agglutinating power of anti-amaryllic serum as a means of separating- 

 B. icteroldes (Sanarelli) from other organisms. He first determines 

 accurately the specific agglutinating doses of the serum, both for the 

 bacillus in question and for the organisms found most frequently in 

 symbiosis with it. Serum is then added, in the former proportion, to 

 a (7 p.c.) gelatin nutrient medium contained in tubes drawn out to a 

 closed funnel-shaped point at the lower end. The tubes are then 

 superficially inoculated with the material to be examined and incubated 



* This subdivision contains (1) Collecting: Objects, including Culture Pro- 

 cesses; (2) Preparing Objects; (3) Cutting, including Imbedding and Microtomes ; 

 (4) Staining and Injecting ; (5) Mounting, including slides, preservative fluids, &c. ; 

 (6) Miscellaneous. 



f Jouin. App. Micr., vi. (1903) p. 2409. % Tom. cit., pp. 2309-14. 



§ Arch. f. Hyg.. xlvii. (1903) p. 57. See Centralbl B;ikt. Kef., xxxiii. (190:!) 

 pp. 665-G. || Centralbl. Bakt. Orig., 1" Abt., xxxiv. (1903) pp. 463-79. 



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