242 J. W. GROVES ON SECTIONS OF ANIMAL TISSUES. 



Dr. Charlton Bastian's method is at follows : — 



Place the sections on portions of tissue, in 

 Gold chloride ... 1 part. 



Distilled water ... 2,000 parts, acidulated with strong 



hydrochloric acid (drop 1 to water 



Let them remain protected from light for an hour, then remove and 

 place them in acidulated water, half the strength of the above, and 

 then transfer to a watch glass containing 



Formic acid. 

 Spirits of wine ^ 



They should be stained in about half-an-hour. 



Or, if it is desired to produce the stain more rapidly, use — 



Chloride of palladium ... 1 part. 

 Water „ ... 500 parts. 



Where they should remain five or ten minutes ; then wash in 

 acidulated water, transfer to the formic acid and spirits of wine, as 

 before, and mount. 



Mr. Schafer's method is the use of the double chloride of gold 

 and potassium. 



1. Place the fresh tissue in bichromate of ammonia (1-2 per 

 cent, in water) solution for fifteen or twenty days. 



2. Dip the sections, after being cut, into — 



a. Water, 1,000 parts. 



b. Double chloride of gold and potassium, 1 part. 



3. Wash in — 



Acid hydrochloric ... ... 1-2 parts. 



Water ... ... ... ... 3,000 parts. 



4. Then immerse for ten minutes in — 



Acid hydrochloric ... ... 1 part. 



60 p.c. solution of alcohol ... 1,000 parts. 



5. Transfer to alcohol absolute. 



6. Clear with oil of cloves. 



7. Mount in balsam or dammar. 



In staining with gold chloride or silver nitrate, Mr. Schafer has 

 found that if the material, after immersion in these stains be dipped 

 into alcohol, the reduction of the metals is thereby greatly facili- 

 tated. 



Osrnic acid, which was first used by Schultze, is useful for the 



