J. W. GROVES ON SECTIONS OF ANIMAL TISSUES. 245 



Then Nicholson's soluble pure blue... gr ^ in 



Alcohol ... ... ... J i, to which (Blue. 



has been added Acid Nit. ... ... my J 



Both solutions should then be filtered. 

 Use n|ij of the blue to 

 nivij of the magenta, 

 and thoroughly mix. 



Place the section in the mixture for about one minute, then 

 remove to absolute alcohol, from that to oil of cloves or benzole, and 

 finally mount in balsam and benzole. 



To fix the magenta, it is usually necessary to pass the sections 

 through benzole. 



Cements, fyc. 



For balsam or glycerine jelly mounts, almost any varnish will do 

 but for fluids or glycerine it is necessary to have one that is tough 

 and most likely to prevent leakage ; for this purpose, the following 

 answers very well : — 



1. Mastic and Bismuth, 



Dissolve gum mastic in chloroform, and thicken with nitrate of 

 bismuth. 



The solution of mastic should be nearly saturated. 



2. Oxide of Zinc, Dammar, and Drying Oil. 



Rub up : — 



Oxide of zinc, well ground, Jij, 

 with Drying oil to consistence of thick paint. 

 Then add an equal part of 



Gum Dammar, dissolved in 

 Benzoline to thickness of syrup. 

 Strain through close meshed muslin. Keep in well corked bottle, 

 and, if necessary, thin with benzoline. 



8. Mr. Kitton's Cement. 



White lead in powder \ 



Red ,, „ I equal parts. 



Litharge ,, ) 



Grind together with a little turpentine, until thoroughly incor- 

 porated, and then mix with gold size. 



The mixture should be thin enough to use with a brush, and in 

 use one coat should be allowed to dry befor applying another. 



