10 



MEMOIRS OP THE NATIONAL ACADEMY OF SCIENCES. 



Agar slant. — A thin greenish white band forms along the line of inoculation by the second 

 day, and the agar has acquired a faint green tint. It soon spreads, reaching the tube wall for the 

 lower third, and has leafy margins. The agar becomes a beautiful yellowish green. 



Gelatin stab. — Growth occurs for some distance down the puncture, and a white button with 

 irregular leafy margins forms on the surface. After several days fine spinous outgrowths form 

 along the puncture, about 1 mm. long and whitish in color. The gelatin becomes 

 faintly green near the surface. There is no liquefaction. 



Potato. — By the secoud day a very thin moist layer about the color of honey has 

 formed over a large part of the surface. After some time this becomes a pale 

 yellowish brown. It remains very thin and has no distinctive features. 



Bouillon. — Becomes diffusely cloudy and an incomplete flaky pellicle forms on 

 the surface. After five days the bouillon becomes a faint greenish tint, and is opaque, 

 whde a white mass forms at the bottom of the tube. 

 \ / Rosolic acid. — Becomes slightly darker after five days. 



X^ .S Litmus milk. — After a week the color becomes more blue, and at the end of four 



Gel. stai.. weeks is a slaty blue. A whitish deposit forms on the bottom of the tube, but there 

 seems to be no change in the milk itself. The reaction is decidedly alkaline. 



Sugar gelatin, deep stab. — Growth quite deep along the puncture, and a button on the surface. 

 There is no liquefaction nor gas production. 

 Indol. — Reaction negative. 



Gelatin slant. — A greenish white band with beaded edges forms along the line of inoculation. 

 The gelatin acquires a green tint about the third day. No liquefaction occurs. 



Relation to temperature. — Grows well at both room temperature and at 35° to 30° C. 



Sarcina Subflava. 



Found at the surface of a cultivated field. 



Character. — No growth in an atmosphere of hydrogen. 



Morphology. — Packets square and longer than broad, showing 4, 8, 1G, 32, or more elements 

 on each face. 



Spore formation not observed. 



Motility. — Non-motile. 



Flagella not demonstrated. 



Colonies on gelatin plates. — Colonies become visible in thirty-six to forty hours as minute 

 yellowish dots, x 80. They are finely granular, yellowish disks with even margins. 

 By the fourth day the surface colonies are 1 mm. in diameter, a pale yellow, and 

 are slightly sunken in the gelatin. x80. They are a pale yellow, homogeneous, 

 and have even margins. After some days the margins may become irregular and 

 look more or less granular. The deep colonies increase very slowly and only change 

 in appearance by becoming more dense. Liquefaction is rather slow. There is 

 nothing distinctive about the colonies at all. 



Agar slant. — A yellowish band, 3 mm. wide, forms by the fourth or fifth day. 

 It is smooth, pale yellow, and has irregular beaded margins. 



Gelatin stab. — Growth quite deep along the puncture, and a small almost white 

 button forms on the surface, which has irregular margins. Slow liquefaction takes 

 place under this in saucer shape. At the end of ten days the liquefactiou is 1 cm. deep and has 

 a level floor. The liquid is cloudy. 



Potato. — No growth could be obtained after a number of trials. 



Bouillon. — Whitish flocculi form at the bottom, and after several days a thin pellicle forms on 

 the surface. The bouillon does not become clouded. 



Rosolic acid. — No change observed. 



Litmus milk. — No change either in color or in the milk itself after four weeks. Reaction 

 faintly acid. 



Sugar gelatin, deep stab. — Growth quite deep and on the surface, with slow liquefaction. No 

 gas production. 



Gel. stab. 



