MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



13 



granules are seen scattered through the colony without any attempt at arrangement. The edges 

 have lost their distinct outline, and the granules are seen invading the gelatin beyond. Rather 

 slow liquefaction takes place. 



Agar slant. — A whitish band, 1 mm. wide, with notched edges forms in twenty-four hours. 

 By the third day this has become 4 mm. wide and spread to the tube wall near the bottom. It 

 is a smooth, white, glistening baud with notched edges, and not very thick. 



Gelatin stab. — By the third day a small saucer of liquefaction has formed, 2 mm. 

 in diameter, while the growth along the puncture is very scanty. Liquefaction is 

 slow, and by the end of two weeks has reached a depth of 1 cm. The floor becomes 

 level and is covered with white floceuli, while the liquefied gelatin is 

 almost perfectly clear. 

 3 Potato. — A moist white band is formed along the line of inoculation. 



/ It spreads widely where moist, and becomes a cream color by the fourth 

 day. Later it spreads all around the plug and changes to a dirty cream 

 color, and is brownish at the edges where it is thin. 



Bouillon. — Diffuse cloudiness is caused in twenty-four hours; a 

 dirty white deposit forms on the bottom of the tube. 

 Rosolie acid. — Becomes slightly darker. 



Litmus milk. — Becomes more blue by the third day, after which the color is 

 rapidly discharged, being gone by the tenth day. There is no coagulation of the 

 Gel. stab. casein. A white deposit collects on the bottom of the tube. 



Sugar gelatin, deep stab. — Crowth confined to upper part of puncture, with liquefaction in 

 saucer shape. No gas is produced. 

 Indol. — Reaction negative. 

 Relation to temperature. — Grows better at room temperature than at 35° to 36° C. 



Bacillus Veuticillatus. 



(Figs. S and II. PI. I. I 



Found at a depth of 7 feet in made soil which had been paved over for a number of years. 



Character. — Good growth in an atmosphere of hydrogen, with extensive liquefaction. 



Morphology. — Thick, straight rods, with rounded ends, from three to five times as long as 

 broad. Forms chains of ten to twelve elements. 



Spore formation observed only in potato cultures after two weeks. They are oval and formed 

 in the center of the rod. 



Motility.— Doubtful; slight, if at all. 



FlageUa not demonstrated. 



Colonies on gelatin plates. — Colonies appear in twelve hours, and in fourteen hours those on 

 the surface have attained a diameter of 1 mm., while the deep are one-fourth of a millimeter, and 

 whitish. The surface colonies are each in a small saucer of liquefaction, with even circular 

 margins. They are grayish in color and translucent, x 80. Deep are grayish looking, and 

 consist of a tangled mass of fine filaments, surrounded by quite a deep fringe of fine lines 

 arranged in a whorl from right to left. The surface colonies are filled with slightly opaque 

 liquefied gelatin, in which fine tangled lines like a ball of thread are seen. At this stage the 

 colonies are indistinguishable from those of the potato bacillus. The edge is fringed with a 

 corona of fine wavy spear points. Liquefaction is rapid, and at the end of twenty hours many 

 of the colonies are 8 mm. in diameter. Each colony soon becomes covered with a thick pellicle, 

 which is a cloudy white, and has irregular ragged edges. 



Agar slant. — A scanty granular looking white band forms along the line of inoculation. At 

 the end of four days it has spread to a width of mm., and become white and smooth in the 

 middle, with irregular, granular edges, looking frosted, as it were. After some time it becomes 

 dirty white, and the agar is colored a faint brown. 



Gelatin stab. — A small saucer of liquefaction has formed in twenty-four hours, and slight 

 whitish growth is seen along the puncture. The liquefaction assumes the funnel form. The 



