MEMOIRS OF THF NATIONAL ACADEMY OF SCIENCES. 21 



Gelatin slant. — An elevated band with even margins forms in thirty-six hours. It becomes 

 elevated, white, and has a greenish, iridescent surface. There is no liquefaction. 



Oelatin stub. — Very slight growth along the puncture. A button forms on the surface, which 

 attains a diameter of 1 mm. It is white, shining, and has irregular, leafy margins. There is uo 

 liquefaction, and the gelatin may become faintly tinged with green near the surface. 



Potato. — A yellowish, moist layer spreads over much of the surface by the third day, and all 

 around the plug at the bottom. It soon becomes a dirty, yellowish brown, is always thin, and has 

 a glossy moist surface. 



Bouillon. — Diffuse, but slight cloudiness is seen by the second day, which increases to some 

 extent, but the growth settles largely to the bottom. After some days the bouillon acquires a 

 faint green tint, and at the end of two weeks is a pale clear green. 



Bosolic acid. — Very slight growth. After three weeks the color has become darker. 



Litmus milk. — Becomes more blue after three or four days. At the end of three weeks it is a 

 deep pure blue. There is no coagulation of the casein or any apparent change in the milk itself. 



Sugar gelatin, deep stab. — A button formson thesurface; no growth along the puncture. There 

 is no gas produced. 



In dot. — Reaction negative. 



Relation to temperature. — Grows better at room temperature than at 35° to 30° C. 



Micrococcus Putatus. 



Found in made soil at a depth of 38 inches. 



Character. — Strict aerobe. 



Morphology. — Large coccus forming irregular groups. 



Spore* formation not observed. 



Motility. — Non-motile. 



Flagella not demonstrated. 



Colonies on gelatin plates. — Colonies appear in from forty to forty-eight hours as minute 

 yellowish dots, which easily escape observation, being translucent. On the surface they are only 

 one-tenth of a millimeter in diamater. x 80. Deep are round, finely granular, and have even 

 edges. Those on the surface are round, with regular edges, and have a yellowish cast. They are 

 finely granular, and have an eccentric nucleus, of slightly darker hue. After Ave days, the deep 

 colonies are still punctiform, while those on the surface are one-half of a millimeter in diameter, 

 and have a greenish yellow color, x 80. Deep have become more dense, but otherwise are 

 unchanged. The surface are yellowish disks, with even distinct edges. No structure can be 

 made out except near the edges, where flue wavy lines radially disposed are seen. The nucleus 

 still remains visible. At the end of a week the colonies are distinctly yellow, but never become 

 larger than 1 mm. Slow liquefaction occurs after two weeks. 



Agar slant. — A beaded line forms along the needle stroke iu twenty-four hours, of a faint 

 yellowish color. It increases rather slowly, and becomes a canary yellow after ten or twelve days. 

 The surface becomes smooth and glistening, and the edges of the band are notched. 



Gelatin slant. — A band 1 mm. wide forms along the line of inoculation, of a yellowish hue. At 

 the end of four days the canary yellow color is well developed. After two or three weeks the 

 gelatin is slowly liquefied, and the growth slips slowly down the shallow groove which is funned. 



Gelatin stab. — Growth is confined to the upper part of the puncture, and by the fourth day 

 a buttou has formed on the surface, which reaches a diameter of i mm. at the end of ten days. It 

 is quite thin, yellow iu color, and has even margins. Liquefaction begius about the twelfth day, 

 and the surface growth becomes concave, then slowly sinks. 



Potato. — A yellow layer is formed by the third day, spreading at the bottom. After ten days 

 a dense, abundant growth has formed, reaching the walls of the tube for the lower half. It is 

 smooth, moist, and shining, and lemon yellow in color. 



Bouillon. — The liquid becomes slightly cloudy by the third day, but the growth is confined 

 almost entirely to the bottom, where it forms granular-looking yellowish masses, not abundant. 



Bosolic acid. — Growth is scanty. Color is somewhat darker after a week. 



