MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 25 



Morphology. — Thick, straight rods with rounded ends, from two to three times as long as 

 broad. Forms chains of considerable length. 



Spore* are large ovals, formed about the center of the rods. 



Motility. — Movements are sluggish. 



Fiagella not demonstrated. 



Colonies on gelatin plates. — Colonics are well advanced in sixteen hours. They are indistinct 

 cloudy masses, with ill-defined margins, resembling the mycelium of a fungus somewhat, x 80. 

 The center is composed of line tangled lines, which send out long filaments in every direction, 

 forming a circular mass. They are much tangled and twisted. At the end of twenty-four hours 

 the colonies are more distinct, and ± mm. in diameter. The gelatin has become softer, and has an 

 iridescent gleam when viewed slantingly. By the third day the colonies are more than 1 cm. in 

 diameter if there be but few on the plate. The gelatin does not pour as when liquefied by the 

 Subtilis, for example, being held in the interstices of the dense net work of fibers, x SO. But 

 little more is seen than on the first day. The fibers have multiplied immensely and are closely 

 matted together, giving a grayish appearance. 



Agar skint. — A moist looking, grayish growth forms in twenty-four hours along the line of 

 inoculation, which has a somewhat felted look. From the edges flue libers run out and soon reach 

 the tube wall. The whole mass thickens, forming a grayish layer, which often wrinkles in the 

 middle. The folds are best seen by looking at the under surface. 



Gelatin stub. — A small saucer of liquefaction is formed on the surface by the second day, and 

 very soon fine filaments are seen boring into the gelatin in every direction, giving the appearance 

 of "an inverted fir tree." A moist, shining skin is formed on the surface, which 

 becomes folded and slowly sinks, a second one forming. The liquefaction becomes 

 complete after some days, and the cloudy masses which floated in the liquid sink to 

 the bottom. 



Potato. — A white granular-looking layer, which covers much of the plug is formed 

 in the course of four days. This becomes much folded and wrinkled if the potato 

 has been freshly prepared. 



Bouillon. — Good growth in the form of veil-like masses, which resemble closely bits 

 of Japanese tissue paper sunk in the liquid. The. bouillon does not become cloudy. 



Eosolic acid. — Color is deepened in thirty hours, and becomes decidedly deeper 



. . Gel. stab. 



m ten days. 



Litmus milk. — Casein is precipitated as a jelly-like mass in twenty-four hours, and the color is 

 slightly darker. The blue color is lost from above downward, the clot becoming the color of milk. 

 It is digested from above downward also, the process being complete in about two weeks. It 

 is then the color of whey, and a blue ring is seen around the tube at the surface. The reaction is 

 decidedly alkaline. 



Sugar gelatin, deep stab.— Growth quite deep down the puncture, with formation of fine 

 outgrowths, as in plain gelatin. No gas is produced. 



Indol. — Reaction negative. 



Relation to temperature. — Grows well at room temperature, and also at 35° to 36° C. 



Bacillus Antenniformis. 



(Figs. 22 and 23, PI. II.) 



Found at the depth of ■"> feet, in virgin soil used as a meadow. 



Character. — No growth in an atmosphere of hydrogen. 



Morphology. — Large, straight rods, with rounded ends, from eight to ten times as long as 

 broad. Occurs singly for most part. 



Motility. — Actively motile. 



Fiagella not demonstrated. 



Spore formation not observed. 



Colonics on gelatin plates. — Colonies are seen in from twelve to fourteen hours as minute 

 colorless dots, x 80. They are slightly oval, yellowish, and granular. From various portions 

 of the periphery, mainly near the poles of the oval, short fine projections are seen, resembling 



