^3 



4 1 2 3_ 



26 MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



the antenna? of insects. At the end of thirty-six hours these can not be seen and the resemblance 

 is lost. At this time the surface colonies are one-fourth of a millimeter in diameter, and are 

 grayish, x 80. They have an orange-brown center, which sometimes shows a granular nucleus- 

 Around it is a coarsely granular zone of the same color, which passes into a fringe of wavy lines, 

 beyond which is a border of colorless markings apparently made up of parallel lines closely 

 packed and folding in and out. It is deeply dentate, and so faint as to be easily overlooked. As 

 the colonies grow older, the granular center encroaches on the zone of the wavy lines and they in 

 turn on the border, which becomes more regular in outline and narrower in proportion to the size 

 of the colony. Each colony causes a saucer of liquefaction on which a pellicle forms, which soon 

 becomes folded. Liquefaction is not very rapid. At the end of a week the colonies are about 

 6 millimeters in diameter. Under 80 not much can be made out except near the edges, where 

 they have a reticular appearance. They are circular and have well-defined edges. 



Agar slant. — On the second day a very thin, barely visible band with irregular margins is 



seen, which near the bottom has spread considerably in leafy shapes. It soon spreads over the 



entire surface of the agar, except near the top. It is very thin, smooth, translucent, and grayish. 



Gelatin stab. — Slight development down the stab near the surface. On the third day a saucer 



of liquefaction 2 mm. in diameter has formed, which soon has a pellicle on its surface. The 



liquefaction reaches the wall of the tube under the surface of the gelatin, leaving a 



ring attached to the tube. The floor becomes level by the tenth day, 1 cm. from 



surface, and has a light deposit of whitish flocculi on it. The liquid is cloudy. 



Further liquefaction is very slow. 



Potato. — "Invisible" on the second day. A moisture only is seen along the 

 line of inoculation, spreading near the bottom to the tube wall. By the third day 

 much of the surface is covered and the layer is thrown into fine folds, which resemble 

 herpetic vesicles at some points. It becomes putty-colored and drier looking, and 

 the folds more numerous. It is always very thin. 



Bouillon. — Growth is never abundant, and soon settles to the bottom, a few 

 GeLstab. ml kes floating on the surface. 



Bosolic acid.— Very slight growth and barely perceptible deepening of the color. 

 Litmus mill: — No change until fifth day, when the color becomes lighter without redness. It 

 is discharged in ten to twelve days, and the milk becomes watery. No coagulation of the casein 

 observed. Reaction faintly acid. 



Sugar gelatin, deep stab. — Growth is scant deep down, and not seen until three or four days. 

 Liquefaction takes place at the surface. No gas produced. 

 Indol. — Reaction negative. 

 Relation to temperature. — Grows well at room temperature, but somewhat faster at 35° to 30° C. 



Bacillus Trommelschlagel. 



Found at the depth of 4 feet in made soil, which had been paved over for several years. 



Character. — Grows fairly well in an atmosphere of hydrogen. 



Morphology. — Slender, straight rods with rounded ends, from five to seven times as long as 

 broad. Occurs singly and also forms short chains. In the separate rods, as in the chains, are 

 seen deeply stained dots, from one to three in each rod. In the chains the line of division between 

 the separate elements is often impossible to make out. 



Spores are formed in eighteen to twenty hours at 35° to 30° C. They are round or slightly 

 oval and formed at the ends, giving the most perfect form of the drumstick. The spore is not 

 exactly at the end, as a small part of the rod, which is usually pointed, projects beyond it. The 

 best specimens are obtained from agar cultures, at room temperature, on the third day. 



Motility. — Movements are active and progressive. Spore-bearing rods are non-motile. 



Flagclla are attached at the poles. 



Colonics on gelatin. — Colonies seen at the end of twenty to twenty-four hours. Deep are 

 whitish and punctiform, and those on the surface but little larger, x 80. Deep are irregularly 

 circular, yellowish, granular disks, with even edges. The surface colonies show a nucleus, usually 



