MEMOIRS OF TIIE NATIONAL ACADEMY OF SCII.M IS 



29 



Gelatin slant. — An elevated porcelain-white band 1 nun. wide, which has a smooth surface and 

 oven margins, is formed by the third day. II is raised one-half of a millimeter. After about ten 

 days indistinct st nations may be seen on the surface of the gelatin running at right 

 angles to the band of growth, but no growth can be made out positively. 



Gelatin stab. — Good growth occurs quite deep, and on the surface a button forms 

 by the second day. It is 1 mm. in diameter and half as high. After a time the edges, 

 whicb are thinner, become corrugated, while the center remains smooth and a glistening 

 pearl white. For some distance down puncture delicate, spinous offshoots 

 are seen, not more than one-fourth of a, millimeter long. There is no 

 liquefaction. 



Potato. — A faintly yellowish line forms along the needle mark by the 

 second day, which has spread widely, where the potato is moist, in a very 

 thin colorless layer. It increases slowly and becomes by the tenth day a 

 dirty white, smooth, moist, and shining layer. 

 Bouillon. — Becomes diffusely clouded by the third day, and a thin film forms on 

 the surface. Later, the growth collects at the bottom of the tube. 



Rosalie acid. — Becomes cloudy, and by the tenth day r is a cherry-red color. 

 Htm us m ilk. — More blue by fourth day. After two weeks changes to a slate-blue, 

 and a whitish deposit forms at the bottom. There is no coagulation or any change 

 apparently in the milk itself. The reaction is strongly alkaline. 



Sugar gelatin, deep stab. — Growth quite deep down the stab and on the surface, 

 produced. 



Indol. — Good reaction with both acid and sodium nitrite. 

 Relation to temperature. — Grows more rapidly at 35° to 36° C. 



7 .lavs. 

 Gel. stall. 



2il .lavs. 

 Si 1 stab, 



No 



gas is 



Bacillus Solitaritjs. 



Found at the depth of -1 feet in made soil, which had been paved for several years. 



Character. — Strict aerobe. No growth in an atmosphere of hydrogen. 



Morphology. — Slender, straight rods, with rounded ends, from three to seven times as long as 

 broad. Occurs singly. 



Spore formation not observed. 



Motility. — Some rotatory non-progressive movements. 



Flagella not demonstrated. 



Colonies on gelatin plates. — Colonies become visible in from thirty-six to forty hours, as grayish 

 dots, x 80. Deep are grayish, smooth-looking disks, with finely notched margins, the contents 

 being homogeneous or sometimes finely granular toward the center. Surface colonies present 

 much the same appearance, but are larger, and some irregular bundles of fine lines can be seen. 

 At the end of forty-eight hours the deep colonies show a nucleus, around which are tolerably well- 

 defined rings or zones, growing lighter toward the edge. Fine wavy lines can be seen running in 

 various directions, giving the colony a somewhat marbled appearance. At the end of seventy 

 hours the surface colonies are 1 mm. in diameter, and of a greenish-white color, with even edges. 

 X 80. A granular nucleus is seen, surrounded by an orange-colored zone, made up of fine lines 

 closely packed. Some are so dense that the lines can not be made out. The edges of this zone 

 are not well defined, and are irregular, sending ragged offshoots into the surrounding zone, which 

 is a lighter shade, and in which wavy lines running toward the edge can be seen. At the end of 

 a week the surface colonies are milk white, round, elevated, and have smooth surfaces and even 

 edges. They are too dense to show any structure under the microscope, except near the margins, 

 where wavy lines are seen. Deep have not changed, except to grow more dense. There is no 

 liquefaction of the gelatin. 



Agar slant. — By the second day a greenish-white line 1 mm. wide has formed. It grows 

 thicker, spreads almost to the tube wall for the lower third, and becomes much whiter, so that by 

 the fourth day it is porcelain white, has irregular margins, and is smooth, moist, and shining. 

 The growth which collects at the bottom becomes cream colored after some days. 



