AIKMOIKS OF THE NATIONAL ACADEMY OF SCIENCES. 



33 



Agar slant. — A whitish translucent band forms along the Hue of inoculation, and by the third 

 day is 3 mm. wide. It is very thin, moist, and lias uneven edges. It seems to be watery, and 

 flows down to the bottom, having a shiny look. It is not tenacious, and but little 

 clings to the needle. About the tenth day whitish masses arc seen, much more dense 

 than the growth. On holding the tube to the light these arc seen to be projections 

 into the agar. They are oval in shape and extend about 1 mm. down. The surface of 

 the agar has a roughened feel to the needle. Quite a quantity of the growth collects 

 iu the water at the bottom, and is milk white in color. 



Gelatin stab. — Growth occurs all along the line of puncture, and a 

 button forms on the surface. In three days this button is one fourth 

 of a millimeter in height and 2 mm. in diameter, and is a porcelain white, 

 smooth and glistening. It does not spread further, but the edges become 

 elevated, forming a shallow basin. The gelatin after a time becomes Agar slant. 

 cloudy on the surface, though no growth can be detected. No liquefaction occurs. 

 Potato. — ( )uly a moisture along line of inoculation is seen at first. It soon spreads 

 in a very thin, colorless layer over much of the surface. After four or five days it 

 becomes a light, dirty brown, smooth, moist, and shining. 



Bouillon. — Growth occurs first at bottom of the tube, but by the third day the 

 Gei.stai.. liquid is diffusely cloudy. Dirty-looking whitish flakes form on the surface, but do 

 not form a pellicle, and sink to the bottom on shaking the tube. The bulk of the growth settles 

 to the bottom as a white mass. 



Bosolic acid. — Becomes much lighter by the fourth day, and is completely decolorized in two 

 weeks. The reaction is alkaline. 



Litmus mill;. — Shows no change until a week has passed, when it becomes darker, and finally 

 changes to an indigo blue, almost. There is no coagulation and no further change seen at end of 

 six weeks. 



Sit (jar gelatin, deep stab. — Growth quite deep down puncture and on the surface. No liquefac- 

 tion and no gas production. 



Gelatin slant. — A porcelain white line 2 mm. wide is formed, which has even, elevated edges 

 and a smooth, glossy surface. The whole surface of the gelatin becomes a cloudy white after 

 some days, but no growth cau be detected. 



Indol. — Good reaction takes place with both sulphuric acid and sodium nitrite. 

 Relation to temperature. — Grows well at room temperature, but somewhat faster at 35° to 

 36° C. 



Bacillus Diffusus (Fraukland). 



Found at the depth of 6 feet in made soil, which had been paved for several years. 



Character. — Shows very slight growth in an atmosphere of hydrogen. 



Morphology. — Slender straight rods, from five to seven times as long as broad. Occurs 

 singly mostly, and in short chains. 



Motility. — Some movement of an oscillatory character, but no progression. 



Flagella not demonstrated. 



Spore formation not observed. 



Colonies on gelatin plates. — Colonies do not become visible until the third day, when they 

 appear as whitish dots, x 80. The deep colonies resemble those of the Comma bacillus, being 

 coarsely granular with irregular margins. Near the edge wavy lines can be made out. The 

 surface colouies have very little color at this stage, being faint bluish green, x SO. They show 

 an indistinct nucleus, are irregularly circular, and have granular contents. As the colouies get 

 older the deep ones reach the surface, and their margins become less distinct, a fringe-like border 

 growing out over the surface of the gelatin. This border has leafy or deeply dentate margins, 

 and is finely granular in structure, while the original colony is darker and very coarsely granular. 

 Liquefaction takes place rather slowly, and each colony is in a saucer-shaped depression. 



Agar slant. — A thin greenish-white layer is developed, which has smooth edges, and is moist 

 and shiuiug. As it increases in thickness it becomes a very pale-yellow color. 

 II. Doc. 253 3 



