38 MEMOIRS. OF THE NATIONAL ACADEMY OF SCIENCES. 



are yellowish granular disks. The colonies slowly increase in size, but seldom get larger than 

 one-halt of a millimeter in diameter. At end of a week a colorless, fringe-like border has formed 

 mound the edge of the surface colonies, which has a leafy outline and is finely veined. The center 

 of the colonies becomes so dense that no structure can be seen. At this time each colony shows a 

 dense center, next a wavy looking zone of lighter brown which fades into a lighter colored and 

 coarsely granular zone with a well-defined border, and lastly the colorless fringe. There is no 

 liquefaction at end of ten days. 



Agar slant.— A greenish- white line about 1 mm. wide forms along the line of inoculation in 

 the course of thirty-six hours. It is slightly elevated, moist, and shining, and in a few days sends 

 out a very thin, colorless layer from the edges, whose margins become very indistinct and almost 

 impossible to locate positively. 



Gelatin stab. — Growth along puncture occurs quite deep, and on the surface a grayish button 

 is formed which attains a diameter of 2.5 mm., and has well-defined margins. After ten days 

 indistinct outgrowths are seen along the puncture quite a distance below the surface. 

 They are globular in shape. No liquefaction of the gelatin was seen at the end of 

 twenty-eight days. 



Gelatin slant.— A white line about 1 mm. is formed, not much elevated, smooth 

 and shining, and with regular margins. No liquefaction took place, though at the 

 end of a month the gelatin felt somewhat soft when touched with a needle. 



Potato. — On second day a faint bluish discoloration is seen along line of 

 inoculation. On third day the growth has spread over a large part of the surface 

 of the plug in a thin yellowish layer, about the color of honey. It is moist and 

 shining, and after two weeks becomes somewhat brown. 



Bouillon. — Diffuse cloudiness is caused, the bulk of the growth finally settling 

 to the bottom in gray masses. 

 Gei.stab. Rosolic acid. — Becomes much lighter, so that at end of two weeks the color 



is entirely discharged. 



Litmus milk. — Becomes a purer blue in twenty-four hours. No coagulation occurs. The milk 

 gradually becomes translucent, and at end of two weeks is a beautiful violet color by transmitted 

 light. This color fades slowly, but is still quite deep at end of six weeks. 



Sugar gelatin, deep stab. — Growth quite deep along stab, with button on surface. No gas 

 production. 



Tndol. — Reaction negative. 



Relation to temperature— Grows well at room temperature, more rapidly at 35° to 36° C. 

 Note. — This bacillus resembles the bacillus Fissuratus in many ways, and seems to be a variety 

 of it. The chief differences are seen in the colonies on gelatin and in its non-liquefaction of gelatin. 

 As the bacillus Fissuratus is a very slow bquefier, a hasty examination would make the two appear 

 identical. 



Bacillus Fissuratus. 



(Figs. 36 and 37, 1*1. III.) 



Found at the depth of 1 foot in made earth, which had been paved for a number of years. 



Character.— Shows very slight growth in atmosphere of hydrogen. 



Morphology. — Small straight rods, two to three times as long as broad, with rounded ends 

 Occurs singly for most part. 



Spores are small, oval, and formed in center of rods. 



Motility. — Only rotatory and oscillatory movements noted, which were active 



Flagella uot demonstrated. 



Colonies on gelatin plates. — Colonies may be seen at end of fourteen hours as minute whitish 

 dots, x 80. No difference can be made out between the deep and superficial colonies at this 

 stage. They are pale yellow, with irregular outline, darker at center, and give one the idea of 

 Hakes of mineral matter, or bits of shell. At the end of two days the surface colonies are 1 mm. 

 in diameter and white, while the deep are still punctiform. x 80. Deep are orange brown and 

 resemble a rosette somewhat. The surface colouies are circular in outline, but somewhat irregular. 



