MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



41 



Spore formation not observed. 

 Mot iliti/. — Non-motile. 



Flaaella not demonstrated. 



Colonies on gelatin plates. — Colonies seen in about thirty hours. The deep are panctiform and 

 white, and under a low power (x 80) resembles masses of amorphous crystals heaped up. They 

 are yellowish brown with very irregular edges. In the body of the colony the edges of the 

 separate masses can be seen overlapping one another, the whole resembling a rosette. At the end 

 of thirty-six hours the surface colonies are one-fourth of a millimeter in diameter, circular, and 

 white, x 80. Appearance much the same as deep, the mass being more dense, and the central 

 structure not so apparent. The center is yellowish brown and coarsely granular, while at points 

 the outlines of the masses composing it can be seen. They became more clearly visible, toward 

 the edge, giving a petal-like arrangement. Beyond is an outer zone which is grayish and finely 

 granular, and fine veins run through it. The colony is circular, with well-defined edges, which are 

 notched here and there. The growth is rather slow, so that at the end of sixty hours the surface 

 colonies are not quite 1 mm. in diameter. At end of eighty-four hours many of them are so dense 

 that it is hard to make out any structure, but in some, by using strong light, the characteristic 

 rosette form can be seen. The deep colonies grow slowly, the masses becoming 

 larger and more regularly disposed, the resemblance to a rosette becoming more 

 marked. In the surface colonies the petal-like arrangement at the edge becomes 

 more distinct, the leaves growing longer and narrower in proportion. As the colony 

 grows older it increases in thickness layer by layer, piling up in the center and 

 growing thinner toward the edges, the color ranging from a dark reddish brown in 

 the center to a pale yellowish gray at the edge. After a week the colonies acquire 

 a fern like border. There is no liquefaction. 



Agar slant. — A white translucent layer is formed which never spreads very 

 widely. It is not much elevated and has leafy edges. After four or five days a 

 very thin, colorless layer is seen around the edges of the first growth, which has 

 ferny outlines. Agar slant. 



Gelatin stab. — Same growth along line of puncture, but mainly on surface, where a thin white 

 layer with irregular leafy edges is formed. It attains a diameter of 4 or •"> mm. 

 No liquefaction at end of three weeks. 



Gelatin slant. — Greenish-white band, '1 mm. wide, elevated, smooth and shining. 

 The edges are irregular, and after some days become finely dentate. 



Potato. — A yellowish layer, moist and shining, which finally covers most of the 

 plug, is formed. At end of a week it becomes a dirty, brownish color - , but no 

 further change occurs. 



Bouillon. — Diffuse cloudiness seen in twenty-four hours. After a few days a 

 thin, friable pellicle forms on surface, while the growth collects at bottom in dense 

 whitish masses. 



Bo8olic «c/r/.— Loses color entirely in six or seven days. 

 Gel - 8ta,) - Litmus milk. — Becomes a pure blue in three or four days. The color is then 



slowly discharged and is entirely lost at end of ten days. The milk becomes watery. No 

 coagulation at any time. 



Sugar gelatin, deep stab. — Growth in upper half of stab, with button on surface. No gas 

 produced. 



Indol. — Reaction negative. 



Relation to temperature. — Grows more rapidly at 35° to 36° C. 



