8 



MEMOIRS OF TI1E NATIONAL ACADEMY OF SCIENCES. 



Blood and venom, equal parts: There is no diminution in the size of the leucocytes. The. 

 only change noticed was a slight stria tion. There was no ameboid movement present. In twenty- 

 four hours the corpuscles were slightly darker aud there was very little diminution in size. In 

 seventy two hours most of the corpuscles still showed good contour. 



Blood and a normal saline solution, equal parts: There was no immediate effect. In seventy- 

 eight hours many of the corpuscles were broken down. 



Blood and a normal saline solution containing 10 per cent venom, equal parts: The erythro- 

 cytes showed slight discoloration. The leucocytes retained their ameboid movement. In forty- 

 eight hours the corpuscles were broken down. 



Blood and a normal saline solution containing 2 per cent venom, equal parts: The erythro- 

 cytes were striated and the ameboid movement still continued. In twenty-four hours all corpuscles 

 were destroyed. 



Dilutions of 0.1 to 0.05 per cent venom in the same way showed the same result as when 2 per 

 cent venom was used. When 0.(15 per cent venom to 0.1 per cent was used, there was no immediate 

 effect whatever upon the corpuscles, and the destruction of the corpuscles occurred in forty-eight 

 hours. 



Pine Snake Blood and Venom of Chotalis Adamanteus. 



THE EFFECT OF VENOM UPON THE BLOOD OF THE CROTALUS ADAMANTEUS. 



The normal erythrocytes of crotalus blood were 25 micromillimeters in the lon^ diameter, 

 oval, and nucleated. The leucocytes were numerous, and many had marked ameboid movement. 

 In twenty-four hours the erythrocytes showed crenation and ameboid movement of the leucocytes 

 had stopped. In ninety-six hours all the corpuscles were broken down. 



When blood and venom were mixed in equal parts the erythrocytes were normal in size but 

 slightly paler, and the ameboid movement was present. Six days later there was no change 

 whatever in appearance of the corpuscles. 



Blood and a normal saline solution, equal parts. The saline solution produced no immediate 

 effect. In ninety-six hours most of the corpuscles were broken down and only a few crenated ones 

 remained. 



Blood and a normal salt solution containing 10 per cent venom, equal parts. There was no 

 immediate effect on the corpuscles. In ninety-six hours there was only a slight crenation. 



