APPENDIX. 



The details of the experiments described above are set forth in the following pages, and 

 include: 



1. Kinds of experimental data and methods for obtaining them. 



2. Statistical details of metabolism experiments with alcohol. 



3. Statistical details of digestion experiments with alcohol. 



4. Tabular summaries. 



A list of the experiments, with groupings for comparison, may be found in Table 1, on 

 page 241 of the first part of this report. As there explained, the metabolism experiments here 

 described in detail were made with alcohol as a part of the diet. They are compared with similar 

 experiments without alcohol, which have been described in detail elsewhere. Each metabolism 

 experiment or series of metabolism experiments with or without alcohol not only included a 

 digestion experiment, but was also preceded by such an experiment. The data of these digestion 

 experiments are also given beyond. The experiments without alcohol and two of those with 

 alcohol have been described in detail elsewhere. In several instances the results are here 

 summarized with the details of the alcohol experiments. 



DATA.-EXPERIMEXTAL METHODS. 

 METABOLISM EXPERIMENTS. 



The larger part of the statistics of the metabolism experiments have to do with the income 

 and outgo of material and energy. 



Experimental <htt<i of income. — These include statistics of the kinds, amounts, composition, 

 and potential energy of food and drink, the volume of the ventilating current of air entering 

 the chamber and the amount of carbon dioxide and water in that air. The food for each 

 experiment was selected before the experiment began and the desired amounts for different 

 meals were placed in suitable jars, as described on page 239. Such of the anatytical determi- 

 nations as were necessary for the control of the diet, in order to insure the desired amount of 

 protein and energ}', were made previous to the beginning of the experiment. 



h'.r/>< rirru ntal data of outgo. — These include statistics of the amount, composition, and heat 

 of combustion of the unoxidized materials of feces and urine, the cpiantity of carbon dioxid 

 and water in the air leaving the chamber, and the total energy given off by the body in the form 

 of heat and external muscular work. 



Apparatus and <j< //< /■>'/ methods of inquiry. — The respiration calorimeter and method of its 

 use have been described in detail in publications referred to on page 236. a The methods of 

 analysis of food, feces, and urine were, in the main, those adopted by the Association of Official 

 Agricultural Chemists. 1 ' but with certain modifications which have been developed in this 

 laboratory. The heats of combustion were determined by use of the bomb calorimeter. d 



"Bulletins 44, 63, 69, arid 109 of the Office of Experiment Stations of the U. S. Dept. Agr. 

 See Bulletin 46, revised, of the Division of Chemistry, U. S. Dept. Agr. 



"See U. S. Dept. Agr., Office of Experiment Stations, Bui. 44, p. 22; Bui. 69, p. IS, ami Report of Storrs 

 (Conn. ) Expt. Sta., 1891, p. 47. The methods for the determination of carbon and hydrogen in use in this laboratory 

 are described in detail by F. G. Benedict in Elementary Organic Analysis, The Chemical Publishing Co., on page 51 

 of which the apparatus is pictured. 



d The bomb calorimeter and accessory apparatus used have been described by W. 0. Atwater and associates in 

 Bulletin 21 of the Office of Experiment Stations of the U. S. Dept. Agr., p. 123, and in the Reports of the Storrs 

 (Conn.) Expt. Sta., 1894, p. 133, and 1897, p. 199. 



289 



