Ch. X] LABELING AND CATALOGUING SLIDES 339 



6. The mechanical treatment, — imbedded, sectioned, dissected 

 with needles, etc. Date at which done. 



7. The staining agent or agents and the time required for staining. 



8. Dehydrating and clearing agent, mounting medium, cement 

 used for sealing. 



9. The objectives and other accessories (micro-spectroscope, 

 polarizer, etc.), for studying the preparation. 



10. Remarks, including references to original papers, or to good 

 figures and descriptions in books. 



§ 528. A catalogue card written according to this formula: 



1. Muscular Fibers of Cat; Cover 0.15 mm.; Fibers 20fx to 40/i 

 thick. 



2. No. 475. (Drr. IX) Oct. 1, 1891. S. H. G., Preparator. 



3. Tendinous and intra-muscular terminations of striated muscular 

 fibers from the Sartor ius of the cat (Felis domestica). 



4. Cat eight months old, healthy and well nourished. Fasting 

 and quiet for 12 hours. 



5. Muscle pinned on cork with vaselined pins and placed in 20 

 per cent nitric acid immediately after death by chloroform. Left 

 36 hours in the acid; temperature 20 C. In alum water {\ sat. 

 aq. sol.) 1 day. 



6. Fibers separated on the slide with needles, Oct. 3. 



7. Stained 5 minutes with Delafield's hematoxylin. 



8. Dehydrated with 95% alcohol 5 minutes, cleared 5 minutes 

 with carbol-turpentine, mounted in xylene balsam; sealed with shellac. 



9. Use a 16 mm. for the general appearance of the fibers, then a 

 2 or 3 mm. objective for the details of structure. Try the micro- 

 polariscope (§ 421). 



10. The nuclei or muscle corpuscles are very large and numerous; 

 many of the intra-muscular ends are branched. See S. P. Gage, 

 Proc. Amer. Micr. Soc, 1890, p. 132; Ref. Hand-book Med. Sci., 

 Vol. V, p. 59- 



§ 529. General remarks on catalogues and labels. — It is especially 

 desirable that labels and catalogues shall be written with some imper- 

 ishable ink. Some form of water-proof carbon ink is the most avail- 

 able and satisfactory. The water-proof India ink, of Higgins or 



