Ch. X] REAGENTS AND THEIR PREPARATION 367 



out with running water 24 hours. Then place in 67 % alcohol 1 day 

 or more and finally preserve in 82 % alcohol. Tissue fixed in Zenker's 

 has mercuric crystals. They may be removed from the tissue by 

 long treatment with iodin, or by putting the slide bearing the sections 

 in iodized alcohol for half an hour or more. 



This is an excellent fixer, combining the good qualities of mercuric 

 chlorid and of the chromium compounds. Tissues fixed with this 

 show well the red blood corpuscles. This is called Helly's fluid if 

 the acetic acid is replaced by 5% formalin. 



Collateral Reading for Chapter X 



Lee, A. B. — The Microtomist's Vade Mecum, 7th ed., 1913. 



Kingsbury, B. F. — Histological Technique, 1915. 



Mann, G. — Physiological Histology, 1903. 



Ehrlich, P., et al. — Enzyklopaedie der Mikroscopischen Technik, 1910. 



Wright, Sir A. E. — Principles of Microscopy, 1907. 



Carpenter-Dallinger. — The Microscope and Its Revelations, 1901. 



Spitta, E. J. — Microscopy, 1907. 



Anatomical Record. 



Journal of the Royal Microscopical Society. 



Transactions of the American Microscopical Society. 



Journal of Experimental Zoology. 



Botanical Gazette. 



Boyer, C. S. — The Diatomaceae of Philadelphia and Vicinity, 1916. 



Dudley and Thomas. — Manual of Plant Histology, 1894. 



Chamberlain, C. J. — Methods in Plant Histology, 1916. 



Stevens, W. C. — Plant Anatomy, 1915. 



Ewart, A. J. — Protoplasmic Streaming in Plants, 1903. 



Bernard, Claude. — Lecons sur les Phenomenes de la Vie communs aux Animaux 

 et aux Vegetaux. Two vols. 1878-1879. 



Needham & Lloyd. — The Life of Inland Waters, 1916. This is a most impor- 

 tant work for all interested in water forms. 



