THE BEHAVIOR OF THE SHORE-ANEMONE 319 



Calcium chloride. — (Saturated solution in sea water, and 3/8 

 normal in distilled water.) With the saturated solution in sea 

 water at the time of injection, forty minutes past nine in the 

 morning, a medium secretion of mucus was produced and a 

 semi-responsive condition immediately upon expanding. At 

 noon the animal was almost normally responsive to contact 

 stimulation. While in the afternoon, the tentacles of the ani- 

 mal had recovered normal responsiveness to contact stimulation, 

 food given them was not accepted. The next day, however, 

 food was slowly accepted in the afternoon, and the succeeding 

 day, the tentacles responded entirely normally when given food. 

 Of the four specimens tested with 3/8 M CaCl in distilled water, 

 two showed a greater mucus secretion than the others and ac- 

 cepted food several hours after; the other two accepted food 

 immediately upon expanding. 



Lithium chloride. — (Normal solution in sea water and 3/8 

 normal in distilled water.) Comparatively little secretion of 

 mucus was produced upon injection. The anemones appeared 

 entirely normal upon expansion, and gave immediate and vigor- 

 ous response to food given to the tentacles. The same effect 

 was produced in the case of the specimens treated with a 3/8 

 M solution in distilled water. 



Magnesium sulphate. — (Normal solution and 3/8 normal in 

 distilled water.) A number of anemones injected with magne- 

 sium sulphate in considerably greater volumes than were used 

 in the preceding experiments showed practically no secretion 

 of mucus, but almost all of them practically total irresponsive- 

 ness to contact stimulation. The tentacles presented a more 

 rigid condition than was the case with the other substances 

 producing a lowered degree of tonus. This is perhaps to be 

 attributed to a partial paralysis of the muscles coupled with 

 a condition of anesthesia in the body of the animal. The ex- 

 periment was begun at ten o'clock in the morning and by two 

 o'clock that afternoon the tentacles contracted after several 

 successive stimulations with a glass rod. At four o'clock in 

 the afternoon of the same day, the tentacles had so far recovered 

 as to become almost normally responsive to contact stimulation, 

 but persistently refused food given to them. Food placed upon 

 the mouth was taken into the gastrovascular cavity. Three 



