APP.] EMBRYO OF THE THIRD DAY. 255 



2. Through auditory sac, shewing: 



a. Auditory sac still open. 



b. The thin roof and thick sides of the hind-brain. 



c. Notochord. 



d. Heart. 



e. Closed alimentary canal. 



3. Through dorsal region, shewing the general appear- 

 ance of a section of an embryo at this stage, which 

 should be compared with a similar section of the 

 earlier stage. 



It shews: 



a. The commencement of the side folds ; the 

 alimentary canal still however open below. 



b. The Wolffian duct lying close under the epiblast 

 on the outside of the protovertebrse. 



c. The notochord with the aortas on each side. 



IV. Examination of an Embryo at the end of the third 



day. 



A. Opening the egg, as in II. A. 



B. Examination of the blastoderm in situ. 



Observe : — 



1. The great increase of the vascular area both in size 

 and distinctness. The circulation is now better 

 seen in situ than after the blastoderm has been 

 removed. 



2. That the embryo now lies completely on its left 

 side and that it is only connected with the yolk-sac 

 by a somewhat broad stalk. 



C. Removal of the embryo. See II. C. 



It is now unnecessary to remove the whole of the 

 blastoderm with the embryo; indeed it is better to 



