260 Cytologie. — Hybriden. — Morphologie. — Physiologie. 



Mixtures of absolute alcohol and bergamot oil were used for 

 Clearing. Gentian violet was used for staining kinoplasm. If 

 the stain washes out too readily, some gentian violet should 

 be dissolved in the absolute alcohol. From two to twenty, 

 seconds immersion in dilute iodide iodine Solution just before 

 transferring to the absolute alcohol often gives a sharper 

 differentiation. 



The summary of Prof. Osterhout's work on spindle for- 

 mation in Agave americana is partly as follows: During early 

 stages in the first division of the pollen mother cell the spindle 

 is enclosed in a special membrane of cytoplasmic origin, which 

 forms a complete investment around it: the functions of the 

 membrane appear to be comparable to those of the nuclear wall 

 and the limiting membrane of the cytoplasm. There is no weft 

 of fibers such as is usually described for this stage. The spindle- 

 forming fibers are radial from the beginning and are attached 

 both to the nuclear and the spindle wall. The second division 

 differs radically from the first, the spindle-formation resembling, 

 in general, that described for the spore-mother-cells of Equl- 

 setum. 



The plates are in three colors, reproducing the effect of 

 the safranin-gentian violet-orange stain. 



Charles J. Chamberlain (Chicago). 



ANONYMUS, Zygonisla X Rolfeana. (Gardener's Chronicle. [3.] 

 Vol. XXXII. 1902. p. 30. Fig. 11.) 



This bigeneric hybrid between Zygopetalum maxillare Gautieri and 

 Aganisia lepida is figured and shortly described. 



H. H. W. Pearson. 



CURTISS, A. H., The yellow water lily of Florida. (Plant 



World. V. p. 106—109. June 1902.) 



Notes on the occurrence and on the two forms of leaves oi Castalia 

 flava, with figure of leai forms. Trelease. 



BESSEY, C. E., Fruits which actas leaves. (Plant World. 



V. p. 111. June 1902.) 



Comparison of the chlorophyll-bearing surface of leaf and fruit of 

 Lepidium apetalum. Trelease. 



Daniel, L, Nouvelles observations sur le greffage et 

 la de'cortication annulaire. (Travaux scientifiques 

 de l'Universite de Rennes. T. I. Fase. I. Rennes 1902. 

 p. 57—63.) 



Au point de vue du grossissement du fruit, le niveau auquel 

 on effectue la de'cortication a son importance. Seuls grossissent 

 les fruits situes dans la region superieure ä Panneau de'cortique ; 

 les autres conservent leurs dimensions normales. C'est d'ailleurs 

 conforme ä ce qui s'observe dans les plantes ligneuses de'corti- 

 qu£es et dans la greffe des fruits. 



