98 SUMMARY OF CURRENT RESEARCHES RELATING TO 



has been sliown that the virus can survive for fifty-one days in blood 

 from a sick animal when this has been freely exposed to the air and 

 allowed to become putrid ; in meat and bones also the virus may persist 

 for many days. 



Further observations are necessary to determine the factors influenc- 

 ing the duration of the vitality of the rinderpest virus in dead animals' 

 tissues. 



(2) Preparing Objects. 



Demonstrating Degeneration of Peripheral Nerves.* — C. Manalong 

 adopted the following technique : — From 2-J: centimetres of the vagus 

 and posterior tibial nerves are laid on a strip of cardboard and treated 

 as follows : 1. Harden in equal parts of Miiller's fluid and formaldehyde 

 (10 p.c.) for twenty-four hours. 2. Replace by Miiller's fluid for fifteen 

 days. 3. Wash in running water for from twelve to twenty-four hours. 



4. Transfer the tissue for fifteen days to the following solution : Miiller's 

 fluid 2 c.cm,, 2 p.c. osmic acid solution 0'5 c.cm., distilled water 0'5 c.cm. 



5. Wash in running water for from twelve to twenty-four hours. 



6. Dehydrate in graded alcohols, changing the absolute alcohol twice. 



7. Clear in origanum oil, tease under a dissecting microscope and mount 

 in chloroform balsam. 



Demonstrating the Cytoplasmic Inclusions of Germ-cells. f — • 

 J. B. Gatenby in his work on the snail used the following fixatives : 



1. Modification of Flemming's strong formula without acetic acid. 



2. Same fluid diluted one-sixth. 3. Champy's fluid. 4. Flemming's 

 strong solution, the acetic acid being replaced by nitric acid. For 

 staining, Ehrlich's hematoxylin and eosin, Mayer's acid ha^malum, iron- 

 haematoxylin, with Orange G, Bensley's acid fuchsin, methyl-green, and 

 alizarin-toluidin-blue. The following was the best of the fixing and 

 staining methods: — 'The animals were anaesthetized in chloroform vapour, 

 -and after the shells were removed the ovotestis is cut out. This is cut 



in half longitudinally, and thrown into Flemming without acetic acid, 

 diluted one-sixth with distilled water. Next day they are washed in run- 

 ning water for two hours, then passed through upgraded alcohol to 

 absolute and xylol. Sections, 6/x, are stained in iron-alum-haematoxylin 

 by the long method — i.e. ten to twelve hours in iron-alum, ten to fourteen 

 in hajmato^ylin. After differentiation the sections are stained with 

 Orange G or van Gieson. The mitrochondria and nebenkern are intense 

 black and beautifully clear. 



C4) staining: and Injecting-. 



Improvised Staining of Malarial Parasites. J — G. Senevet used 

 the following improvised eosin-azure stain for malarial blood, in the 

 absence of special stains for this purpose : — 



Two solutions are used : 1. Methylen-blue 1 gram, sodium borate 



* Philippine Journ. Sci., xii. (1917) p. 171. 



t Quart. Journ. Micr. Sci., Ixii. (1917) pp. 562-4. 



J Bull. Soc. Path. Exeta, x. (1917) pp. 540-2. 



