ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 101 



survived on cane-sugar and 21 p.c. on beer- wort ; of the second only 

 one in nine survived on cane-sugar for more than 7 ' 5 years, but all five 

 cultures on beer-wort survived for twenty-five years. Only two out of 

 five cultures of Schizosaccharomyces on cane-sugar survived, but ten out 

 of eleven of those on beer- wort were living. Of six cultures of 

 A. glaucus only one survived, and two of the remaining five perished in 

 less than two years. 



(6) Miscellaneous. 



Rapid Method for obtaining HaBmolytic Serum.* — A. Sezary 

 recommends the following rapid method : — Into the peritoneal sac of a 

 rabbit a single injection of washed sheep's-corpuscles is made. The 

 ■quantity of red corpuscles is that which is found in 85 c.cm. of de- 

 fibrinated blood. The animal is bled eight days afterwards. The 

 hEemolytic'serum is just as powerful as if the animal were injected four 

 or five times. 



Detection of Bile-pigments in Blood-serum.f — A. Fouchet obtains 

 blood by venous puncture (5 c.cm.). The serum is obtained by centri- 

 fuging or by leaving it to exude. Five drops are placed on some white 

 surface, and then a similar quantity of the following reagent is added : 

 Trichloracetic acid, 5 grm. ; water, 20 c.cm. ; perchloride of iron, 

 officinal, 2 c,cm. The two are thoroughly mixed with a glass rod. In less 

 than twenty minutes a colour is obtained ; this is greenish-blue and 

 stable. If there be -^-q^^-q of bihration the colour comes out at once. 



* C.R. Soc. Biol. Paris, Ixxx. (1917) pp. 797-8. 

 t C.R. Soc. Biol. Paris, Ixxx. (1917) pp. 826-8. 



I 



