ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 667 



and dried at 37° C. The dry pigment is fairly soluble in methyl-alcohol, 

 and more so if 5 p.c. glycerin be added. To make up the stain for use 

 it is advisable to pound up 0"25 grm. with some sharp clean sand : this 

 prevents the stain going into a sticky mass when the alcohol is added. 

 Then dissolve the dry residue in methyl-alcohol containing 5 p.c. glycerin. 

 Stain for 5 minutes or more in a solution made with 2 parts stock 

 solution and 1 part distilled water. Rinse in distilled water. Fix for 

 | to 1 minute in Unna's glycerin-ether mixture 2 p.c. in H 2 0. Rinse in 

 distilled water. Differentiate and dehydrate in absolute alcohol. Any 

 precipitate is easily removed by means of methyl-alcohol, or by equal 

 parts of absolute alcohol and xylol. Then xylol and balsam. This stain 

 does not appear to act well after osmic acid or its mixtures, but good 

 results are obtainable from sublimate audits mixtures. 



Savini, E., & Th. Savini-Castano — Zur Technik der Elastika und Binde- 

 gewebs-farbung. Zeitschr. wiss. Mikrosk., xxv. (1909) pp. 29-47. 



Sommerhoff, E. 0. — Die Farbung der Pikrinsaure auf Seide. 



A phenomenon of Osmosis, in which the sheath of the silk filament acts 

 as an animal membrane ; chromaturgical considerations in reference to 

 the staining of bacteria. Tom. cit., pp. 48-51. 



Cavazza, L. E. — Studi microchemici e fisiologici sui Tamiini. 



Tom. cit., pp. 59-64. 



(5) Mounting-, including - Slides, Preservative Fluids, etc. 



Examining and Mounting the Digestive System of Schizopoda.* — 

 C. Gelderd fixed the material in the following fluid : — Formalin, 40 p.c, 

 5 c.cm. ; alcohol, 94 p.c, 30 ccm., H L ,0 100 ccm. ; or in Gilson's 

 fixative : Nitrate of copper, 200 gr. ; formalin, 500 ccm. ; sea-water, 

 200 c.cm. ; 7 ccm. were used with 100 c.cm. of sea-water. The 

 sections were stained and mounted in the usual way, or with Gilson's 

 cuparal, which has the advantage of being able to be used after de- 

 hydrating in 70 p.c. alcohol, t 



Isobutyl-alcohol was very useful when mounting in cuparal, as it is 

 less volatile and absorbs water less rapidly than ethyl-alcohol, and delicate 

 objects could be manipulated by its aid without danger of contracting 

 the tissues. The procedure was as follows :— Dehydrate with ethyl-alcohol 

 at 70 p.c ; wash in isobutyl-alcohol ; mount immediately in cuparal. 



(6) Miscellaneous. 



Metal Filter.t. — E. Gobbi describes a filter, made of nickel, the pores 

 of which are fine enough to hold back ultra-microscopic particles. 

 Such an apparatus should be invaluable in bacteriological laboratories. 



Don, J. — The Filtration and Purification of Water for Public Supply. 



Proc. Inst. Mech. Eng., 1909, pp. 1-209 (3 pis.) 



* La Cellule, xxv. (1909) pp. 7-68 (4 pis.). 



t See this Journal, 1906, p. 501. 



X Comptes Eendus, cxlviii. (1909) pp. 1126-8. 



