ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 66] 



B. Technique.* 

 (1) Collecting- Objects, including 1 Culture Processes. 



Bacillus typhosus and B. coli on media containing- Blood and 

 Carbohydrates.f— E. P. Bernstein has observed the different behaviour 

 of 1). typhosus and II. coli when grown on agar containing 1 p.c. of 

 various (16 varieties) carbohydrates, to each 15c.cm. of which 1 c.cm.of 

 sterilised ox blood has been added. On lactose blood agar typhoid 



colonies produced no haemolysis ; coli colonies did. On raffinose hi 1 



agar typhoid colonies are umbilicated with radiating lines ; coli colonies 

 do not show these peculiarities. On maltose blood agar typhoid colonies 

 arc almost black ; coli colonies are dull white. On dextrose blood agar 

 typhoid colonies caused a precipitation and were of a black colour ; coli 

 colonies caused haemolysis and were wliite. 



Special Nutrient Medium for Cholera Vibrio.f — A. Dieudonne 

 finds that alkaline blood agar is a favourable medium for the cholera 

 vibrio and is antagonistic to the growth of B. coli and other intestinal 

 organisms. The medium is prepared by mixing defibrinated blood with 

 an equal amount of normal caustic potash and sterilising ; 30 parts of 

 this solution are then added to 7o parts of nutrient agar prepared in the 

 usual way. 



Cultivation of Bacillus Leprae^— M. T. Clegg describes attempts 

 to grow the bacillus of leprosy in combination with an amoeba and its 

 symbiotic bacterium on a, medium composed of agar 20, sodium chloride 

 0*3, beef-extract 0*3. A culture of amoebae was obtained from a 

 dysentery stool, and after a sufficient growth of the amoeba? had 

 occurred to over-balance the symbiotic bacteria, leprosy bacilli were 

 added by smearing the surface of the media with pulp from a leper's 

 spleen. A short, plump, acid-fast bacillus developed, and from this sub- 

 cultures were made. Successful results were obtained from two different 

 cases of leprosy. 



Fixation Methods and the Swelling of Alga Membranes.|| — 

 F. Tobler makes an attempt at estimating the magnitude of the errors 

 arising out of certain fixation methods and the amount of swelling of 

 alga membranes. The materials used were the youngest portions of the 

 red alga Polysiphonia. The fluids experimented with" were : — (1) Iodine 

 in sea-water. (2) Merkel's Solution (equal parts 1-400 platinum chloride 

 and 1-400 chromic acid made up with water or sea-water). (3) 

 Flemming's weak solution. (4) Formalin 40 p.c. diluted with water or 

 sea-water. (5) Saturated solution of picric acid in 50 p.c. alcohol. 



Demonstrating Karyokinesis in Stypocaulon Scoparium.t — E. 

 Escoyez fixed the material in Bouin's or Flemming's fluid ; the latter 



* This subdivision contains (1) Collecting Objects, including Culture Pro- 

 cesses ; (2) Preparing Objects ; (3) Cutting, including Imbedding and Microtomes ; 

 (4) Staining and Injecting; (5) Mounting, including slides, preservative fluids, etc. ; 

 (6) Miscellaneous. 



t Centralbl. Bakt., lte Abt. Orig., 1. (1909) p. 1. J Tom. cit., p. 107. 



i< Philippine Journ. Sci., iv. (1909) pp. 77-9. 



|| Zeitschr. wiss. Mikrosk., xxvi. (1909) pp. 51-8. 



1 La Cellule, xxv. (1909) pp. 1S1-201 (1 pi. and 30 figs.). 



Oct. 20th, 1909 2 y 



